品牌 Beyotime碧云天 产品名称 活性氧试剂盒 产品规格 >100次 型号 S0033S 英文名称 Reactive Oxygen Species Assay Kit 交易保障 买家保障 卖家承诺履约合规诈骗保赔,保障商品交易安全 价格说明 价格:商品在爱采购的展示标价,具体的成交价格可能因商品参加活动等情况发生变化,也可能随着购买数量不同或所选规格...
An ROS assay kit was provided by Beyotime (Wuhan, China). Crystal violet was purchased from Sigma (Saint Louis, USA). The work solution of NAC (Beyotime, China) was 100 mM diluted by deionized water. Cell culture The human breast tumor cell line MDA-MB-231 (MDA231) was obtained ...
Using a BCA assay kit (Beyotime, China), we measured the protein concentration of the supernatant. Liver samples were separated by SDS-PAGE (10%) and transferred onto a PVDF membrane using a wet Trans-Blot system (Bio-Rad, USA). After sealing, the membrane was incubated with primary and ...
The cellular ROS levels was measured using the ROS assay kit (Beyotime, Shanghai, China) as previously reported [58]. Cells were plated overnight on coverslips in a 6-well plate, the cells were washed with PBS after treatment with the specified drugs, and then incubated with serum-free med...
碧云天生物技术/Beyotime Biotechnology 订货热线:400-168-3301或800-8283301 订货e-mail :order@ 技术咨询:info@ 碧云天网站 微信公众号 网址: 活性氧检测试剂盒 产品编号 产品名称 包装 S0033 活性氧检测试剂盒 100次 产品简介: 活性氧检测试剂盒(Reactive Oxygen Species Assay Kit,也称ROS Assay Kit)是一...
BCA Protein Assay Kit (beyotime, Jiangsu, China) was utilized for quantifying the extracted proteins. The prepared proteins were denatured at 98 °C for 6 min after being mixed with sodium dodecyl sulfate (SDS) loading buffer. By SDS-polyacrylamide gel electrophoresis (SDS-PAGE) usage, the ...
The protein concentration was determined with BCA Protein Assay Reagent Kit (Beyotime Biotechnology, Shanghai, China). Approximately 40 μg of each protein sample was separated by SDS-PAGE, then transferred onto PVDF membranes (Millipore Corporation, Bedford, MA, USA). The membranes were blocked...
Prostate cancer cell lines stimulated in vitro as indicated above were lysed in the cell lysis buffer (Beyotime Biotechnology, China). The lysated were quantified with the bicinchoninic acid (BCA) kit (Beyotime Biotechnology, China). Then, the supernatants were subjected to 10% SDS-PAGE and then...
Intracellular ROS level was determined with ROS assay kit (Beyotime, Shanghai, China) according to the manufacturer's instructions. Briefly, cells were equipped with 10 mM DCFH-DA 30 min before radiation. Upon entry into cells, DCFH-DA is hydrolyzed by cellular esterase into DCFH, which then ...
Cell proliferation assay. Cell proliferation was measured by the CCK-8 assay kit (Beyotime, China). Briefly, cells were seeded in 96-well culture plates the day before isoliensinine treatment. After incuba- tion, 10 μ l of CCK-8 reagent was added to each well and the absorbance...