Nrf2 is anchored by Kelch-like ECH-associated protein 1 (Keap1) in the cytoplasm[[[22]]]. However, in the case of oxidative stress, the Nrf2-Keap1 interaction dissociates, and activated Nrf2 enters the nucleus, forms a
The intracellular ROS was determined by ROS assay kit (Solarbio, Beijing, China). In brief, cells in different treatment groups were harvested, rinsed with PBS and incubated with DCFH-DA at 37 °C for 30 min. Then the DCF fluorescence of the cells was detected by fluorospectrophotometer ...
In brief, the cells were treated with different concentrations of thioridazine and curcumin alone or in combination. After 24 h, the XTT assay was employed to measure the cell viability using a WelCount Cell Viability Assay Kit (WelGENE, Daegu, Korea). Reagent was added to each well and ...
After the indicated treatments, the total protein of IVD cells was extracted using a Western and IP cell lysis kit (Beyotime, China). The protein concentrations were measured using a BCA protein assay kit (Beyotime, China). Equal protein amounts (30 μg) were resolved on 10–12% SDS-PA...
158 and 43 kDa marked below the blots, were determined by Gel Filtration Calibration Kit HMW (GE Healthcare). The loading inputs for gel filtration were shown below (b).cDimer LDHA presents the noncanonical enzyme activity. The canonical and noncanonical LDHA enzyme activity assays were measur...
propidium iodide (PI) staining after 75% alcohol fixation was used, followed by flow cytometric analysis. Cell death was determined by flow cytometry after the cells were double stained with Annexin V-FITC and PI using an assay kit from BD Pharmingen (San Diego, CA, USA) in a routine manne...
Schematic illustration of the antibacterial applications of h3-FNCs with density effect. Single Fe atoms distribute densely in the high-loading single-atom catalysts (h3-FNCs), leading to neighboring Fe–N4moieties being close enough to each other, and resultantly, the largely enhanced intrinsic oxida...
(Beyotime Biotechnology, China). Briefly, 100 mg HA-CS/PDA hydrogels with different PDA NPs concentrations were immersed in 1 ml RPMI-1640 for 12 h. The hydrogel extracts were tested using a total antioxidant capacity assay kit (Beyotime Biotechnology, China) according to the manufacturer...
ALKandROS1molecular analysis ForALKandROS1fusion analysis, DNA and RNA were extracted from paraffin-embedded sections using the KingFisher kit according to the manufacturer’s instructions. The concentration of extracted DNA and RNA was measured using NanoDrop 2000c Spectrophotometer (Thermo Fisher Scient...
The present invention relates to exendin-4 peptide analogues which selectively bind and activate the glucagon receptor and comprise a chelating moiety capable of binding a metal ion and their use, for example in PET imaging.Inventors: Haack, Torsten (Frankfurt am Main, DE) Plettenburg, Oliver ...