Western blot analysis was performed as follows: The liver tissues were ground using a tissue grinder after the addition of RIPA Lysis Buffer (Beyotime, China). Using a BCA assay kit (Beyotime, China), we measured the protein concentration of the supernatant. Liver samples were separated by SDS...
HO· scavenging ability of PDA NPs was evaluated by detecting fluorescent 2-hydroxyterephthalic acid formed by terephthalic acid oxidation. O2·−scavenging ability of PDA NPs was evaluated by nitro blue tetrazolium (NBT) assay (Beyotime Biotechnology, China). The total antioxidant capacity of hydroge...
The beyotime Institute of Biotechnology (Shanghai, China) offered ROS assay kit, BCIP/NBT alkaline phosphatase chromogenic kit, and Alizarin red-S staining solution. R&D Systems (MN, USA) offered recombinant mouse RANKL and M-CSF for the experiment. The Cell Signaling Technology (MA, USA) ...
Prostate cancer cell lines stimulated in vitro as indicated above were lysed in the cell lysis buffer (Beyotime Biotechnology, China). The lysated were quantified with the bicinchoninic acid (BCA) kit (Beyotime Biotechnology, China). Then, the supernatants were subjected to 10% SDS-PAGE and then...
碧云天生物技术/Beyotime Biotechnology 订货热线:400-168-3301或800-8283301 订货e-mail :order@ 技术咨询:info@ 碧云天网站 微信公众号 网址: 活性氧检测试剂盒 产品编号 产品名称 包装 S0033 活性氧检测试剂盒 100次 产品简介: 活性氧检测试剂盒(Reactive Oxygen Species Assay Kit,也称ROS Assay Kit)是一...
For ROS assay, BMMNCs were treated with or without different doses of HQ for 2 h, and subsequently incubated with CD34-ECD (Beckman Coulter, Indianapolis, IN, USA) and DCFH-DA for 10 min at 37°C using a ROS Assay Kit (Beyotime Biotechnology, Shanghai, CN) and then, the intrace...
Cell viability assay An MTT Cell Proliferation and Cytotoxicity Assay Kit (Beyotime Biotechnology, Shanghai, China) was used to determine cell viability. HCT116 and SW480 cells (6 × 103cells per well) were seeded into 96-well plates and incubated overnight at 37 °C. After that, HC...
The lactate dehydrogenase cytotoxicity assay kit was purchased from Beyotime (C0016). According to the kit instructions, cells in the logarithmic growth phase were seeded into a 96-well plate and treated with different concentrations of APG-115. Additional wells were reserved for culture medium witho...
The H2O2 level was determined with the Hydrogen Peroxide Assay Kit (Beyotime Biotechnology, Shanghai, China). The medium treated with NTP was harvested immediately and mixed with the detecting buffer. The absorbance of the supernatant was measured at 560 nm with the Varioskan Flash microplate rea...
The ROS Assay Kit (BestBio Company, China) was utilized in compliance with the guidelines provided by the manufacturer. A 50 mg sample of fresh flap tissue was homogenized and centrifuged. After that, 190 μL of the supernatant and 10 μL of the ROS probe were combined, and the mixture ...