Western blot analysis was performed as follows: The liver tissues were ground using a tissue grinder after the addition of RIPA Lysis Buffer (Beyotime, China). Using a BCA assay kit (Beyotime, China), we measured the protein concentration of the supernatant. Liver samples were separated by SDS...
HO· scavenging ability of PDA NPs was evaluated by detecting fluorescent 2-hydroxyterephthalic acid formed by terephthalic acid oxidation. O2·−scavenging ability of PDA NPs was evaluated by nitro blue tetrazolium (NBT) assay (Beyotime Biotechnology, China). The total antioxidant capacity of hydroge...
碧云天生物技术/Beyotime Biotechnology 订货热线:400-168-3301或800-8283301 订货e-mail :order@ 技术咨询:info@ 碧云天网站 微信公众号 网址: 活性氧检测试剂盒 产品编号 产品名称 包装 S0033 活性氧检测试剂盒 100次 产品简介: 活性氧检测试剂盒(Reactive Oxygen Species Assay Kit,也称ROS Assay Kit)是一...
Prostate cancer cell lines stimulated in vitro as indicated above were lysed in the cell lysis buffer (Beyotime Biotechnology, China). The lysated were quantified with the bicinchoninic acid (BCA) kit (Beyotime Biotechnology, China). Then, the supernatants were subjected to 10% SDS-PAGE and then...
For ROS assay, BMMNCs were treated with or without different doses of HQ for 2 h, and subsequently incubated with CD34-ECD (Beckman Coulter, Indianapolis, IN, USA) and DCFH-DA for 10 min at 37°C using a ROS Assay Kit (Beyotime Biotechnology, Shanghai, CN) and then, the intrace...
Cell viability assay An MTT Cell Proliferation and Cytotoxicity Assay Kit (Beyotime Biotechnology, Shanghai, China) was used to determine cell viability. HCT116 and SW480 cells (6 × 103 cells per well) were seeded into 96-well plates and incubated overnight at 37 °C. After that, ...
The ROS Assay Kit (BestBio Company, China) was utilized in compliance with the guidelines provided by the manufacturer. A 50 mg sample of fresh flap tissue was homogenized and centrifuged. After that, 190 μL of the supernatant and 10 μL of the ROS probe were combined, and the mixture ...
Western blotting assay Prostate cancer cell lines were treated in vitro as indicated and lysed in the cell lysis buffer (catalog no. P0013B; Beyotime Biotechnology, China) presented with protease /phosphatase inhibitor cocktail (catalog no. 5872; Cell Signaling Technology, Danvers, MA). The lysate...
Intracellular ROS assay. Intracellular ROS generation was detected using DCFH-DA (2,7-dichlorodihydrofluorescein diacetate, Beyotime Institute of Biotechnology, China). Briefly, harvested cells were washed twice by serum-free medium, loaded with 10 μM DCFH-DA and incubated in cell ...
(DAF-FM DA), Hematoxylin & Eosin (H&E) Staining Kit, paraformaldehyde solution, Mitochondrial Membrane Potential Detection Kit, Cell Cycle Analysis Kit, and TdT-mediated dUTP Nick-End Labeling (TUNEL) Apoptosis Assay Kit were obtained from Beyotime Institute of Biotechnology (Shanghai, China). An...