Memory storage includes a short-term phase (STM) which requires the phosphorylation of pre-existing proteins, and a long-term phase (LTM) which needs the novel synthesis of RNA and proteins. Cyclic AMP and a specific transcription factor (cAMP response element binding protein or CREB) play a ...
Given that a large number of factors can influence nucleosome positioning, its organization is highly diverse and varies considerably from gene to gene and location to location8,9. As a result of this, predicting nucleosome organization accurately at specific locations is still an extremely difficult...
using the human embryonic kidney (HEK293T) cells, which are useful for evaluation of the transactivation activity of hematopoietic cell-specific transcription factors. Luciferase activity driven by the shortest promoter (−136/+71) was significantly augmented with ectopic PU.1 to a similar ...
The transcription factors of the ETS family are involved in the control of the endothelial-specific expression of genes that are important for the formation of new blood vessels. The analysis of the expression pattern of ets1, the gene inactivation of tel and fli1, the in vitro analysis of ...
Total RNA was extracted from the cells using TRIzol®Reagent (Life Technologies, Carlsbad, CA), and 2μg of RNA were used to synthesize cDNA using the HelixCript™ first-strand cDNA synthesis kit (NanoHelix, Korea). cDNA was obtained by PCR amplification using β-actin-specific and Sp1-...
- 《American Journal of Pathology》 被引量: 127发表: 2010年 The stem cell factor SOX2 regulates the tumorigenic potential in human gastric cancer cells Aberrant expression of the specific gastric differentiation marker SOX2 has been observed in stomach cancer. However, the role of SOX2 in gastric...
Transcription factor AP2 and its role in epidermal-specific gene expression. A Leask,C Byrne,E Fuchs - 《Proceedings of the National Academy of Sciences》 被引量: 380发表: 1991年 The interaction of specific peptide aptamers with the DNA binding domain and the dimerization domain of the ...
7). BAF53B expression in neurons is known to be mediated by the downregulation of the RE1-Silencing Transcription factor (REST), a negative regulator of neuron-specific genes20. In prostate adenocarcinoma cells, we observed that short-term REST knock-down led to an increase of BAF53B (ACTL6B...
Blimp-1 promoted the migration and adhesion of plasmablasts. It directly repressed genes encoding several transcription factors and Aicda (which encodes the cytidine deaminase AID) and thus silenced B cell–specific gene expression, antigen presentation and class-switch recombination in plasmablasts. It...
In addition, the role of cytosine methylation of the promoter region has been analyzed to elucidate whether this epigenetic mechanism might contribute to control the specific regulation of the expression of this gene. Results Features of the 5' flanking region of the human podocalyxin The human ...