Responses and adaptation under abiotic stress require differential gene expression, which is regulated by specific transcription factors. Transcription factors are proteins with a DNA domain that binds to the cis-acting element present in the promoter of a target gene. This chapter gives an overview ...
Given that a large number of factors can influence nucleosome positioning, its organization is highly diverse and varies considerably from gene to gene and location to location8,9. As a result of this, predicting nucleosome organization accurately at specific locations is still an extremely difficult...
The transcription factors of the ETS family are involved in the control of the endothelial-specific expression of genes that are important for the formation of new blood vessels. The analysis of the expression pattern of ets1, the gene inactivation of tel and fli1, the in vitro analysis of ...
Transcription factor AP2 and its role in epidermal-specific gene expression. A Leask,C Byrne,E Fuchs - 《Proceedings of the National Academy of Sciences》 被引量: 380发表: 1991年 The interaction of specific peptide aptamers with the DNA binding domain and the dimerization domain of the ...
using the human embryonic kidney (HEK293T) cells, which are useful for evaluation of the transactivation activity of hematopoietic cell-specific transcription factors. Luciferase activity driven by the shortest promoter (−136/+71) was significantly augmented with ectopic PU.1 to a similar ...
Almost 20 years after its discovery, multiple combinations of transcription factors have been introduced into cells to screen for cell-type-specific sets of transcription factors, as in the case of iPSC induction. After much effort, defined transcription factors that enable the direct induction of ...
In addition, the role of cytosine methylation of the promoter region has been analyzed to elucidate whether this epigenetic mechanism might contribute to control the specific regulation of the expression of this gene. Results Features of the 5' flanking region of the human podocalyxin The human ...
Transcription factors function to regulate gene transcription. They may be constitutively expressed or may only be activated during specific situations. Activator protein-1 (AP-1) is an inducible transcription factor, and is comprised of multiple protein complexes that include the gene products of the...
Total RNA was extracted from the cells using TRIzol®Reagent (Life Technologies, Carlsbad, CA), and 2μg of RNA were used to synthesize cDNA using the HelixCript™ first-strand cDNA synthesis kit (NanoHelix, Korea). cDNA was obtained by PCR amplification using β-actin-specific and Sp1-...
7). BAF53B expression in neurons is known to be mediated by the downregulation of the RE1-Silencing Transcription factor (REST), a negative regulator of neuron-specific genes20. In prostate adenocarcinoma cells, we observed that short-term REST knock-down led to an increase of BAF53B (ACTL6B...