Pannexin 1 (Panx1) is the most widespread member of the pannexin family of proteins and its mRNA expression has been detected in all bone cells1,2,3. Similar to connexins, Panx1 molecules associate to form hemichannels in the cell membrane, but unlike connexins, there is no evidence for ...
Advanced prostate cancer initially responds to hormonal treatment, but ultimately becomes resistant and requires more potent therapies. One mechanism of resistance observed in around 10–20% of these patients is lineage plasticity, which manifests in a p
Myocardial tissue was used to extract the samples, which were then boiled in a loading buffer for five minutes. 10% SDS-PAGE was used to separate the proteins. Primary antibodies were grown on the PVDF membranes overnight at 4 °C. The primary antibodies used in this study were Actin (Dil...
The total cell extracts from induced and non-induced cells were analysed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). A band corresponding to 30 kDa (Fig. 4) was detected in induced cells carrying the expression plasmid pMLH27, but was absent from induced cells ...
(26). The amount of radioactivity in bands separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)1 was quantitated by scanning the dried gels using an AMBIS β scanner. Preparation and Fractionation of Cell Lysates For RcsA fractionation experiments, cells were grown in M6...
Samples were boiled at 96°C for 5 min and the proteins were separated on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane in a wet tank unit (Bio-Rad) at 60 V by using blot buffer (16 ...
2002. A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell 14:2723–2743 PubMed CAS Google Scholar Chiwocha SDS, Abrams SR, Ambrose SJ, Cutler AJ, Loewen S, et al. 2003. A method for profiling classes of ...
Posted inHydroxylase, 11-?? A score of 0 was taken into consideration negative, a score of just one 1 was taken into consideration positive weakly, and a score of two or three 3 was taken into consideration positive strongly 1479-5876-8-135-S1 ...
Using 1-D SDS–PAGE and nano-LC–MS/MS analysis, Alin et al.104 identified several exosomal proteins from the ascites of three CRC patients and found that some of these proteins may play roles in tumor development by disrupting epithelial polarity and affecting metastasis, proliferation, immune ...
1c). To exclude false negatives, all investigated GSTs were confirmed to be solubly and functionally produced by SDS–PAGE analysis and an activity test with the generic GST substrate CDNB (5) (Extended Data Fig. 3f,g). Biochemical characterization of PtGSTF8 To characterize the enzymatic ...