High-molecular-weight glutenin subunits (HMW-GS) composition was analyzed by sodium-dodecyl-sulfatpolyacrilamid-gel electrophoresis (SDS-PAGE), while the quantitative determination of total HMW-GS was obtained by reversed phase- high performance liquid chromatography (RP-HPLC). Considering HMW-GS ...
Their purity was confirmed by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Oriole staining (Fig. 3a). Then the RNAs were extracted from the purified virions and subjected to NGS analysis. We obtained two datasets containing 70,281 and 90,911 reads ...
For each mock and receptor condition, 40 or 90 μg of protein was loaded in 10% (v/v) acrylamide gels for SDS-PAGE. Proteins were transferred onto PVDF membranes using the Trans-Blot SD semidry transfer cell (Bio-Rad) at 15 V for 18 min. After blocking overnight, membranes were ...
The purity of the sample was confirmed as single band (>90%) by SDS–PAGE. 2.4. SPR analysis A binding assay was performed using BIAcore X (GE Healthcare). The full-length 4EBP2 or its residue-deleted mutant, as a ligand molecule, was immobilized on a CM5 sensor chip by the amine-...
Equal amounts of protein (40 μg) were firstly separated by 10% SDS-PAGE and transferred onto PVDF membrane. After blocking for nonspecific binding (5% nonfat milk in TBS containing Tween20 (TBS-T)) at room temperature for 1 h, the membranes were incubated with Matrix metallopeptidase ...
Myocardial necrosis and edema were quantified after manual planimetry both on the delayed contrast enhancement and T2-weighted images by delineation of myocardium with signal intensity 4 SDs above the mean signal obtained in the remote noninfarcted myocardium using MASS 7.6 analysis software. If ...
Revealing states (think of a modal as a “state”) are particularly useful because we can play some slight-of-hand tricks that make everything seem very fast. We can lazy-load the hidden state after the rest of the page is done, but keep it dormant and paused, so that it can easily...
State-dependent strategies (SDSs) are the most general form of harvest policy because they allow the harvest rate to depend, without constraint, on the state of the system. State-dependent strategies that provide an optimal harvest rate for any system state can be calculated, and stochasticity ...
Reactions were carried out at 30°C for different times up to 60 min, and at the end of the incubation period, half of the reaction mixture was spotted onto P81 paper, while the other half was trichloroacetic acid-precipitated and analyzed by SDS-PAGE. Human recombinant COX-1 and COX-2 ...
Pellets were washed in (in mM) 50 Tris HCl (pH 8.0), 10 EDTA, and 100 PMSF; repelleted (25,000 × g, 15 min); resuspended in same buffer; and 30 μg of this membrane protein was denatured in SDS loading buffer and resolved by SDS-polyacrylamide gel electrophoresis (PAGE) (NR2A,...