What is the purpose of microscopy labs and steps? What is the purpose of fossae in the head and from what are they created? What is the purpose of the soft agar? Explain. How do SDS PAGE and Immunodetection work? What is the application (purpose) of MSA? Why doesn't healthy urine ...
SDS-PAGEblot分析单克隆抗体重叠延伸PCR高通量筛选c-mycIn this study,a multipurpose M13 KE phage display vector was constructed from wild-type M13 KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the ...
Why do we use SDS PAGE gel instead of agarose for protein separation? Why are some bands darker in gel electrophoresis? What does the electric current do in gel electrophoresis? Why use a negative control when running gel electrophoresis?
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The GST–hOCTN2 polypeptide was collected in the inclusion bodies and showed an apparent molecular mass on SDS-PAGE of 85 kDa. After solubilization with a buffer containing 0.8% sarkosyl and 3 M urea, the fusion protein was applied onto a Ni2+-chelating chromatography column. The ...
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When do you use SDS-PAGE, column chromatography, or immunoprecipitation? How do you know which one to use when testing proteins? If catalase enzyme was placed in a test tube and heated to 100^oC, what results would you expect from a g...