1c). To exclude false negatives, all investigated GSTs were confirmed to be solubly and functionally produced by SDS–PAGE analysis and an activity test with the generic GST substrate CDNB (5) (Extended Data Fig. 3f,g). Biochemical characterization of PtGSTF8 To characterize the enzymatic ...
a H2O2-induced STAT3 oxidation is diminished upon depletion of AnxA2 in HEK293 MSR cells. The formation of covalent (disulfide-linked) STAT3 dimers and tetramers in response to increasing concentrations of exogenously applied H2O2 is visualized by immunoblotting on non-reducing SDS-PAGE. b The sa...
1c). To exclude false negatives, all investigated GSTs were confirmed to be solubly and functionally produced by SDS–PAGE analysis and an activity test with the generic GST substrate CDNB (5) (Extended Data Fig. 3f,g). Biochemical characterization of PtGSTF8 To characterize the enzymatic ...
Samples containing equal amounts of protein were resolved by SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% Difco skimmed milk (BD, Shanghai, China) and then incubated with primary and secondary antibodies. The antibodies used in...
A, cell lysates (30 μg of protein) were subjected to SDS-PAGE Western blotted for phosphorylation of SphK1 and SphK2 and total SphK1 and SphK2. Shown is a representative blot of three independent experiments in triplicate. Note the increase in SphK1 phosphorylation at 10, 15, and 30 ...
2002. A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell 14:2723–2743 PubMed CAS Google Scholar Chiwocha SDS, Abrams SR, Ambrose SJ, Cutler AJ, Loewen S, et al. 2003. A method for profiling classes of ...
Another possibility is that other DNases exist in the Tetrahymena mitochondria (E. Osada, personal communication), as identified using SDS-DNA PAGE [22]. Although these DNases are unlikely to either interact with AIF or to be major effectors of PND, they may contribute to the retarded DNA ...
Cell pellets and supernatants were stored at -70°C until assayed for their content of cytokines or proteolytic activity. SDS-PAGE and western blot analysis Supernatants were concentrated (10×) using Centricon (YM-10) centrifugal filter devices (Millipore, Bedford, MA). For Western blot analysis...
PAGE | 3 The Role of Critical Minerals in Clean Energy Transitions Executive summary Executive summary PAGE | 4 The Role of Critical Minerals in Clean Energy Transitions Executive summary In the transition to clean energy, critical minerals bring new challenges to energy security An energy system ...
For Western blot, equal concentration of tissue lysates were loaded onto the gel and separated on a sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) at 100 V in electrophoresis buffer (25 mM Tris, 190 mM Glycine and 0.1% SDS). The proteins were separated and transferred to PVDF membrane...