Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa. Analysis...
1c). To exclude false negatives, all investigated GSTs were confirmed to be solubly and functionally produced by SDS–PAGE analysis and an activity test with the generic GST substrate CDNB (5) (Extended Data Fig. 3f,g). Biochemical characterization of PtGSTF8 To characterize the enzymatic ...
The total cell extracts from induced and non-induced cells were analysed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). A band corresponding to 30 kDa (Fig. 4) was detected in induced cells carrying the expression plasmid pMLH27, but was absent from induced cells ...
Samples were boiled at 96°C for 5 min and the proteins were separated on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane in a wet tank unit (Bio-Rad) at 60 V by using blot buffer (16 ...
Then, purified rHc-PBS-5 was concentrated using hyperspeed centrifugation method and analyzed by SDS-PAGE. The protein was inoculated subcutaneously into rabbits to produce the polyclonal antibody of Hc-PBS-5 (4 immunizations, 5 weeks apart). A pre-bleed was taken from each rabbit prior to ...
of gallbladder carcinoma. Regarding the prognostic benefit of CA15-3, we might affirm how the sensitivity of the marker can be more pronounced in comparison to MIB-1 and CD34. p75. On the other hand an appreciable immunoreactivity for p75 was seen in the specimens gathered from individuals ...
Samples were removed at intervals, and analyzed by 10% SDS-polyacrylamide gel electrophoresis (PAGE) followed by immunoblotting using the anti-FtsH serum and detected by ECL (Amersham Pharmacia Biotech). Western Blotting 1 ml of culture (Klett unit = 50) was harvested by centrifugation. Pelleted...
(SDS-PAGE) and transferred to a 0.45 μM polyvinylidene fluoride (PDVF) membrane (Merck Millipore). The membrane was blocked with 5% skimmed milk (Merck Millipore) in Tris-buffered saline containing 0.1% Tween-20 for 1 h at room temperature (RT) and probed with the primary antibodies: TAZ...
Using 1-D SDS–PAGE and nano-LC–MS/MS analysis, Alin et al.104 identified several exosomal proteins from the ascites of three CRC patients and found that some of these proteins may play roles in tumor development by disrupting epithelial polarity and affecting metastasis, proliferation, immune ...
We previously elucidated principles for designing ideal proteins with completely consistent local and non-local interactions which have enabled the design of a wide range of new αβ-proteins with four or fewer β-strands. The principles relate local bac