Glioblastoma (GB) is a highly invasive type of brain cancer exhibiting poor prognosis. As such, its microenvironment plays a crucial role in its progression. Among the brain stromal cells, the microglia were shown to facilitate GB invasion and immunosupp
Isolation of RNA, DNA and quantitative PCR Total RNA was extracted from colon tissue using TRIzol reagent (Thermo Fisher). The high capacity cDNA synthesis kit (Thermo Fisher) was used for synthesis of cDNA. Relative amounts of mRNAs were measured using polymerase chain reaction (PCR) and StepO...
Briefly, macrophages were washed with ice-cold PBS, and cellular cholesterol was extracted with 800 μl of ice-cold hexane:isopropanol (3:2, v/v) with butylated hydroxytoluene (50 μg/ml) added in hexane and incubated for 30 min on ice with occasional gentle mixing. The mixture was taken...
A total of 6067 genes in the Labrenzia sp SY1 and 6067 genes in Labrenzia sp PO1 were identified by RNA sequencing. Totally, 2402 genes out of 6067 were found as differentially expressed genes (DEGs). PO1 strain, 595 with decreased transcript abundance, and 1807 increase transcript abundance...
Forty-two µL of the extract was used for targeted amino acid analysis. The tissue pellet was subjected to a second extraction step with 10%/90% dichloromethane-isopropanol at a 1:30 w/v sample-solvent ratio. For the relative quantification of metabolites by both GC/MS and LC-MS/MS, ...
30 w/v sample-solvent ratio. Forty-two µL of the extract was used for targeted amino acid analysis. The tissue pellet was subjected to a second extraction step with 10%/90% dichloromethane-isopropanol at a 1:30 w/v sample-solvent ratio. For the relative quantification of metabolites by...
000 rpm for 5 min. Nucleic acids were precipitated by adding 260 μl of 10 M ammonium acetate and one volume of isopropanol. The pellets were then washed with 70% ethanol and suspended in TE buffer. RNA was removed by treatment with 2 μl of DNase-free Rnase (10 mg/ml) at 37 °C...
The CHRM2 gene, located on the long arm of chromosome 7 (7q31-35), is involved in neuronal excitability, synaptic plasticity and feedback regulation of acetylcholine release, and has been implicated in higher cognitive processing. The aim of this study is the identification of functional (non...
A number of crop wild relatives can tolerate extreme stress to a degree outside the range observed in their domesticated relatives. However, it is unclear whether or how the molecular mechanisms employed by these species can be translated to domesticated crops. Paspalum (Paspalum vaginatum) is a ...
RNA isolation and PCR analysis. Total RNA was isolated using Trizol reagent according to the manufacturer's instructions. First-strand cDNA synthesis from 5 mg of total RNA was performed using SuperScript II reverse transcriptase primed by random hexamer primer. The transcripts of G0s2, PPARg2, C...