Glioblastoma (GB) is a highly invasive type of brain cancer exhibiting poor prognosis. As such, its microenvironment plays a crucial role in its progression. Among the brain stromal cells, the microglia were shown to facilitate GB invasion and immunosuppression. However, the reciprocal mechanisms by...
Libraries were screened using RNA-sequencing (RNA-seq) to reveal the differentially expressed genes (DEGs) involved in anthocyanin biosynthesis at the transcriptional level. Comparison with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database shows that a total of 27 unigenes participate in ...
Main Methods: We assessed the expression of Mettl3 in liver tissues of diabetes (db/db) mice, obese (ob/ob) mice, high saturated fat-, cholesterol-, and fructose-induced non-alcoholic fatty liver disease (NAFLD) mice, and alcohol abuse and alcoholism (NIAAA) mice by quantitative reverse-t...
The CHRM2 gene, located on the long arm of chromosome 7 (7q31-35), is involved in neuronal excitability, synaptic plasticity and feedback regulation of acetylcholine release, and has been implicated in higher cognitive processing. The aim of this study is the identification of functional (non...
Finally, the algae dissolving ability of bacteria was observed under the microscope. 2.3. Extraction of RNA and cDNA library preparation TRIzol (Life Technologies, Thermo Fisher Scientific Inc.) reagent was used to extract the total RNA from PO1 (16 h log and 32 h stationary phase) and SY1...
After 24 hours the cells were pelleted and re-suspended in 1 mL TRIreagant RT (Molecular Research Center) for RNA extraction and synthesis of the immune library. cDNA library synthesis Double-stranded cDNA (dscDNA) libraries were syn-thesised from poly(A)+ RNA extracted from the female ...
2.7. RNA extraction, cDNA synthesis and quantitative real-time PCR In order to measure the relative expression levels of target mRNAs, all cell lines were seeded into 6-well plates and treated with EPSs of probiotic yeast strains. After incubation at 37 °C for 24 h, total RNA was ext...
Forty-two µL of the extract was used for targeted amino acid analysis. The tissue pellet was subjected to a second extraction step with 10%/90% dichloromethane-isopropanol at a 1:30 w/v sample-solvent ratio. For the relative quantification of metabolites by both GC/MS and LC-MS/MS, ...
Hepatocellular carcinoma (HCC) is a common malignant tumor with a complex immune evasion mechanism posing a challenge to treatment. The role of the S100A10 gene in various cancers has garnered significant attention. This study aims to elucidate the impac
Mental health disorders(MHD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) have been widely studied. However, the underlying role of inflammatory cytokines and their associated signaling pathways have not been investigated. Here, we report the potential role of cytokines and associated...