A number of crop wild relatives can tolerate extreme stress to a degree outside the range observed in their domesticated relatives. However, it is unclear whether or how the molecular mechanisms employed by these species can be translated to domesticated crops. Paspalum (Paspalum vaginatum) is a ...
Mycoplasma detection and control is determined every 15 days of culture. Total RNA was extracted by Tri- zol™(Invitrogen) according to the manufacturer' instruc- tions, with an additional overnight precipitation step at -20°C with isopropanol (Merck). RNA quantification and purity were carried...
Hepatocellular carcinoma (HCC) is a common malignant tumor with a complex immune evasion mechanism posing a challenge to treatment. The role of the S100A10 gene in various cancers has garnered significant attention. This study aims to elucidate the impac
ATP citrate lyase (ACLY) and acyl-CoA short-chain synthetases 2 (ACSS2) are key enzymes in lipid metabolism. We explored the role of ACLY in gastric cancer (GC) and the effect of ACLY and ACSS2 compensation on GC growth. We used immunohistochemistry to verify the expression level of ACLY...
Analysis of DAG and ceramide content DAGs and ceramides were extracted by the use of an extraction mixture composed of isopropanol:water:ethyl acetate (35:5:60; v/v/v). Quantitative measurement was made using an Agilent 6460 triple quadrupole mass spectrometer. Diacylglycerols and ceramides were ...
RNA was pelleted by centrifugation at 15,800 × g at 4°C for 30 minutes and then dissolved in the denaturing solution, to which an equal volume of 100. isopropanol was added for reprecipitation of RNA at −20°C. After centrifugation, the RNA pellet was washed with 75% ethanol twice...
The circPVT1 locus is embedded in the long non-coding RNA PVT1 and it originates from exon 2 of the PVT1 gene (human genome GRch38/hg38). HNSCC is the sixth leading cancer by incidence worldwide and the eighth most common cause of cancer death [26, 27]. Although in the past two...
RNA extraction and quantitative reverse transcriptase PCR Total RNA was extracted from the mouse liver at the same anatomical position using TRIzol Reagent (TaKaRa) and reverse-transcribed into cDNA using the Reverse Transcription System (TaKaRa). qRT-PCR was performed using the SYBR Green qPCR Master...
Glioblastoma (GB) is a highly invasive type of brain cancer exhibiting poor prognosis. As such, its microenvironment plays a crucial role in its progression. Among the brain stromal cells, the microglia were shown to facilitate GB invasion and immunosuppression. However, the reciprocal mechanisms by...
Mental health disorders(MHD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) have been widely studied. However, the underlying role of inflammatory cytokines and their associated signaling pathways have not been investigated. Here, we report the potential role of cytokines and associated...