相较于RNase P，RNase MRP催化的底物主要为核糖体rRNA前体，催化切割pre-rRNA 中ITS1区域的A3位点，进而促进核糖体的加工成熟。在该项工作中，雷鸣团队从酵母中成功提取RNase MRP复合物，利用冷冻电镜单颗粒重构技术，分别解析了RNase MRP全酶（2.5Å）和带有底物的复合物（2.9 Å）高分辨率结构，该结构清晰揭...
RNase MRP has distinct substrate specificities, primarily involved in ribosomal RNA biogenesis, but also cleaving mitochondrial RNA and mRNAs involved in cell cycle control. We review current information regarding the nuclear RNase P and RNase MRP enzymes in the eukaryotes, focusing on the relationship...
相较于RNase P,RNase MRP催化的底物主要为核糖体rRNA前体,催化切割pre-rRNA 中ITS1区域的A3位点,进而促进核糖体的加工成熟。在该项工作中,雷鸣团队从酵母中成功提取RNase MRP复合物,利用冷冻电镜单颗粒重构技术,分别解析了RNase MRP全酶(2.5Å)和...
此次蛋白结构解析沙龙第二期中,来自上海交通大学附属第九人民医院精准医学研究院的周斌博士将为大家分享核糖核酸酶MRP蛋白质结构研究方法与进展;来自德国IBA Lifesciences的研发总监Dennis Karthaus博士将为大家分享基于Strep-tag®技术平台开发单域抗体的流程。讲座期间,达科为公司也准备了精美的礼品、试用福利、蛋白质研究...
【Science | 雷鸣团队首次阐释核酶RNase MRP的催化机理】RNase MRP是存在于所有真核生物中一类保守的由一条非编码RNA催化亚基和近十个蛋白质组成的核糖核酸复合物。在组成成分和进化上,RNase MRP都与体内另一类保守且必需的核酶RNase P密切相关。但是二者具有完全不同的底物特异性和底物识别机制,功能上也不具有冗余性...
(Fig.5b) is evolutionarily conserved in terms of both the primary sequence and known structures across all kingdoms of life (Extended Data Fig.10), and is therefore of functional importance. Mutations in the pseudoknot core of human mitochondrial RNA processing RNase (RNase MRP) are linked to ...
(Fig.5b) is evolutionarily conserved in terms of both the primary sequence and known structures across all kingdoms of life (Extended Data Fig.10), and is therefore of functional importance. Mutations in the pseudoknot core of human mitochondrial RNA processing RNase (RNase MRP) are linked to ...
RNase MRP is an essential eukaryotic ribonucleoprotein complex involved in the maturation of rRNA and the regulation of the cell cycle. RNase MRP is related to the ribozyme-based RNase P, but it has evolved to have distinct cellular roles. We report a cryo-EM structure of theS. cerevisiaeRNas...
RNase MRP is a ribonucleoprotein complex with a site‐specific endonuclease activity. Its original substrate for cleavage is the small mitochondrial RNA near the mitochondrial DNA replication origin, thus it was proposed to generate the primer for mtDNA replication. Recently, it has been shown to ...
RNase P and RNase MRP Shortly after the identification of the group I intron inTetrahymena,theRNAcomponent ofRNaseP was also shown to function as aribozyme.RNase Pis an RNA-protein complex that cleaves pre-tRNAs at the 5′ end of the mature tRNA sequence in all organisms. The bacterialenzyme...