Peptide synthesis can take place directly on RNA, which suggests how a nucleic acid–protein world might have originated on early Earth. Felix Müller ,Luis Escobar &Thomas Carell Article|04 May 2022 Selective inhibition of miRNA processing by a herpesvirus-encoded miRNA ...
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cDNA synthesis was performed with SuperScript II reverse transcriptase (Thermo Fisher Scientific) with random hexamer (QIAGEN) or oligo (dT)12-18 (Thermo Fisher Scientific) as primers. Gene-specific reverse transcription was primed with Taqman probes (Applied Biosystems). qPCR was done using Taqman...
The non-segmented negative strand (NNS) viruses use a distinct RNA capping pathway (Figure 2a). The most studied NNS, VSV, codes for a large (L) protein performing both replication/transcription and capping ofviral RNA[15]. The cap synthesis is ensured by a polyribonucleotidyltransferase (...
(Fig.6). RNA editing, as a co/posttranscriptional modification, can change nucleotide sequences and have important effects on viral replication, protein synthesis, infection, and virulence. ADARs and APOBECs, in addition to deamination, are RNA-binding proteins that directly interact with endogenous...
? RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing. ? Synthesis of capped RNAin vitrowith addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA ...
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence ...
The molecular events underlying the assembly and maturation of the early pre-60S particles during eukaryotic ribosome synthesis are not well understood. Here, the authors combine yeast genetics and biochemical experiments to characterise the functions of two important players of eukaryotic ribosome biogenesi...
processing and actuation of specific signals into defined functions and have yielded programmable biological systems and novel therapeutics of increasing sophistication. However, challenges centred on expanding the range of analytes that can be sensed and adding new mechanisms of action have hindered the ...
Taken together, these data suggest that protection of HT-29 cells from radiation-induced apoptosis was facilitated by COX-2 mediated prostaglandin synthesis. CUGBP2 Is an AU-Rich RNA Binding Protein CUGBP2 is an RNA binding protein that binds to predominantly AU-rich sequences Good et al. 2000...