Profiling a pooled CRISPR screen targeting various biological processes, we show the gene expression regulation during RNA synthesis, processing and degradation, miRNA biogenesis and mitochondrial mRNA processing, systematically decoding the genome-wide regulatory network that underlies RNA temporal dynamics ...
Metabolic labeling of newly transcribed RNA for high resolution gene expression profiling of RNA synthesis, processing and decay in cell culture. J. Vis. Exp. http://dx.doi.org/10.3791/50195 (2013). Wisniewski, J.R., Zougman, A., Nagaraj, N. & Mann, M. Universal sample preparation ...
? RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing. ? Synthesis of capped RNAin vitrowith addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA ...
and for both control and experimentally treated cell types. A constitutively expressed "housekeeping" gene would appear to be a good model for an internal standard RNA. Unfortunately, there is no one single RNA with a consta...
plant signal trafficking, involving plant phloem packed with RNA molecules; and RNA transport where the RNA transcript is assembled into a ribonucleoprotein fibril, compacted into a ring-like structure, that gradually unfolds through the nuclear pore to be finally ...
In recent years, single-cell analyses have revealed the heterogeneity of the tumour microenvironment (TME) at the genomic, transcriptomic, and proteomic levels, further improving our understanding of the mechanisms of tumour development. Single-cell RNA
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Bottom right: Fib-RIP RNA-seq and poly(A)− RNA-seq revealed sno-lncRNA with snoRNA ends; E: a model of SPA processing 随着snoRNA大量被鉴定, 研究人员发现, 一些RNA虽然具有box C/D或者box H/ACA的典型结构, 但却找不到能与rRNA或者snRNA相匹配的互补序列, 这类分子被称为孤儿snoRNA(orphan ...
and sending commands to the sequencing device, they critically differ in the signal-processing approach used to identify molecules. RISER performs a binary classification of the raw signal for a given RNA class, while AS basecalls and maps in-progress reads to a predefined list of target ...
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