The chemical synthesis of RNA offers the unique possibility to introduce site-specific modifications and attachment sites for biophysical labels. In this chapter, chemical modification strategies for RNA are summarized, and a brief overview is provided of the incorporation of modified oligonucleotides into...
Summary. Several oligoribonucleotide fragments which are related to the C4N hypothesis, were synthesized by both the liquid and solid phase methods via the phosphotriester approach, in which the combination of the 2-Thp group with the 5-DMTr group was used for chain elongation.doi:10.1080/...
The RNA fragment synthesis of (I)-(III), using ′perfectly′ protected monomer units such as (IVa)-(IVd), and the synthesis of ribo-oligonucleotides (V), by the solid-phase method, are described.doi:10.1002/chin.198638313T. HATAM. SEKINE...
A number of nucleotide phosphoramidites are now available that permit the chemical synthesis of RNA, modified RNA and RNA-DNA chimeric oligonucleotides. Since the chemical strategy allows the introduction of a particular modification at any given site in a nucleotide polymer, very subtle and specific...
Chemical Synthesis of a 5'-Terminal TMG-Capped Triribonucleotide m32,2,7G5'pppAmpUmpA of U1 RNA The 5'-terminal TMG-capped triribonucleotide, m32,2,7G5'pppAmpUmpA, has been synthesized by condensation of an appropriately protected triribonucleotide de... Mitsuo,Sekine,Michinori,... - 《Journ...
They are mainly of three types: (i) Messenger RNA: The most important function of mRNA is that it carries genetic codons (made up of three nucleotides), that result in the coding of the amino acids that results in the synthesis of proteins. (ii) Transfer RNA: Their main function is...
To address this problem, a chemical and an enzymatic approach have been investigated to introduce the selenium functionality into both DNA and RNA covalently. In the chemical approach, the solid phase synthesis via the phosphoramidite chemistry was utilized to synthesize relatively short oligonucleotides....
Chemical synthesis occurs when two or more molecules are combined to form a product molecule that possesses specific properties.
During synthesis of the first DNA coding for a human peptide hormone (angiotensin II) and some other oligodeoxynucleotides e.g. the recognition sequence for the restriction endonuclease Pst I some chemical improvements using diester and triester method for the chemical synthesis of oligodeoxynucleotides...
and express genomic information. It consists of two forms, Deoxyribonucleic Acid (DNA) and Ribonucleic Acid (RNA). DNA encodes the information that cells need to make proteins, while RNA, in different molecular forms, performs a variety of roles in the cell, including the synthesis of proteins...