Wachowius, FalkRes. Group Nucleic Acid Chem., MPI Biophys. Chem., D‐37077 Goettingen, GermanyWILEY‐VCH VerlagCheminformHöbartner C, Wachowius F (2010) Chemical synthesis of modified RNA. In: Mayer G (ed) The chemical biology of nucleic acids, vol 1. Wiley, Chichester, UK, pp 1–...
A number of nucleotide phosphoramidites are now available that permit the chemical synthesis of RNA, modified RNA and RNA-DNA chimeric oligonucleotides. Since the chemical strategy allows the introduction of a particular modification at any given site in a nucleotide polymer, very subtle and specific...
The RNA fragment synthesis of (I)-(III), using ′perfectly′ protected monomer units such as (IVa)-(IVd), and the synthesis of ribo-oligonucleotides (V), by the solid-phase method, are described.doi:10.1002/chin.198638313T. HATAM. SEKINE...
RNAs are central to all gene expression through the control of protein synthesis. Four major nucleosides, adenosine, guanosine, cytidine and uridine, compose RNAs and provide sequence variation, but are limited in contributions to structural variation as well as distinct chemical properties. The ability...
, chemical synthesis of a 5'-terminal TMG-capped triribonucleotide m3 2,2,7G5'pppAmUmA of U1 RNA K Seio 被引量: 26发表: 1996年 Chemical Synthesis of a 5'-Terminal TMG-Capped Triribonucleotide m32,2,7G5'pppAmpUmpA of U1 RNA The 5'-terminal TMG-capped triribonucleotide, m32,2,7G5'...
The invention relates to a method for the chemical synthesis of RNA, comprising the following steps: a) bonding to a solid support of a monomer having formula (II) in which—Xis a dimethoxytrityl group,—Xis H or an OAc group or OX, in which Xis a group having formula (A), in whi...
The demand for large, position-specific modified RNA molecules is high across diverse fields. Now a DNA polymerase has been engineered to enable the efficient and flexible synthesis of such molecules using a pause–restart strategy. This methodology can be implemented in both liquid and hybrid solid...
Summary. Several oligoribonucleotide fragments which are related to the C4N hypothesis, were synthesized by both the liquid and solid phase methods via the phosphotriester approach, in which the combination of the 2-Thp group with the 5-DMTr group was used for chain elongation.doi:10.1080/...
摘要: Current methodologies used to synthesize DNA and RNA are reviewed. These focus on using controlled pore glass and microarrays on glass slides.关键词: controlled pore glass DNA nucleic acid chemical synthesis polymer support RNA synthetic nucleic acid ...
synthesis. Therefore, a need exists to understand the influence of modified nucleosides on conformational dynamics of the ribosome under solution conditions that mimic the cellular environment. In this review on chemical probing, we provide detailed protocols for the use of dimethyl sulfate (DMS) to ...