Investigations by Feulgen microspectrophotometry in Paramecium aurelia indicated that as fission age increased the amount of macronuclear DNA decreased. It was also found that the amount of RNA synthesis as determined by the in vivo incorporation of [ 3H]uridine decreased as the fission age increased...
We have compared RNA polymerase promoter activities in PCR-generated DNA fragments for use in the in vitro transcription of cRNA probes. Sense oligonucleotide primers, specific for the mouse acidic fibroblast growth factor gene, were synthesized with 5' extensions containing promoter sequences for the...
The G1 and G2 phases involve the synthesis of RNA and proteins but not of DNA. • The S phase involves the replication of DNA. • The M phase involves the separation of the chromosomes during cell division. Progression through the cell cycle is controlled by cyclins, proteins whose conc...
摘要: Current methodologies used to synthesize DNA and RNA are reviewed. These focus on using controlled pore glass and microarrays on glass slides.关键词: controlled pore glass DNA nucleic acid chemical synthesis polymer support RNA synthetic nucleic acid ...
The sites of RNA , processing and migration were studied with light and microscope radioautography in adrenal fasciculata of the young . were injected iv with [3H]and sacrificed at various time intervals. A marked radioactive peak was found in blood at the 20 min interval, later time intervals...
Fig. 1: Enzymatic conversion of DNA microarrays to RNA. aThe three-step process of conversion consists of 1- primer hybridization followed by crosslinking, 2- T7 RNA polymerase-mediated RNA extension, and 3- DNase-mediated degradation of the DNA template.bApplication of the DNA-to-RNA method...
In vivo and in vitro (tissue slices) incorporation of labeled precursors into DNA, RNA, and proteins was measured in mitochondria obtained from cerebral hemispheres, cerebellum, and brain stem of rats at different days of postnatal development. To compare the synthesis of macromolecules in mitochondri...
Fig. 5: Delivery of the megabase synAC by programmed haploidization. Full size image Properties of hosts harboring the synAC Fig. 6: Properties of host harboring the synAC. Full size image Data availability The whole-genome sequencing and RNA-seq reads are available on NCBI BioProject (PRJNA...
may inhibit the synthesis of bacterial proteins, DNA and RNA, leading to bacterial death. tty.com.tw 膜電位的消失導致抑制細菌蛋白質、 DN A和RNA的合成,引起細菌死 亡。 tty.com.tw [...] packaging signal (ψ, psi) and the polypurine tract (ppt), which serves as the site of initiation...