Investigations by Feulgen microspectrophotometry in Paramecium aurelia indicated that as fission age increased the amount of macronuclear DNA decreased. It was also found that the amount of RNA synthesis as determined by the in vivo incorporation of [ 3H]uridine decreased as the fission age increased...
The G1 and G2 phases involve the synthesis of RNA and proteins but not of DNA. • The S phase involves the replication of DNA. • The M phase involves the separation of the chromosomes during cell division. Progression through the cell cycle is controlled by cyclins, proteins whose conc...
We have compared RNA polymerase promoter activities in PCR-generated DNA fragments for use in the in vitro transcription of cRNA probes. Sense oligonucleotide primers, specific for the mouse acidic fibroblast growth factor gene, were synthesized with 5' extensions containing promoter sequences for the...
may inhibit the synthesis of bacterial proteins, DNA and RNA, leading to bacterial death. tty.com.tw 膜電位的消失導致抑制細菌蛋白質、 DN A和RNA的合成,引起細菌死 亡。 tty.com.tw [...] packaging signal (ψ, psi) and the polypurine tract (ppt), which serves as the site of initiation...
XI. Structure of RNA-linked DNA fragments of Escherichia coli The size of RNA attached to nascent DNA fragments of Escherichia coli with a chain length of 400 to 2000 nucleotides is estimated to be about 50 to 100 nucleotides from: (a) the density of the molecules of known sizes; (b) ...
Fig. 1: Enzymatic conversion of DNA microarrays to RNA. aThe three-step process of conversion consists of 1- primer hybridization followed by crosslinking, 2- T7 RNA polymerase-mediated RNA extension, and 3- DNase-mediated degradation of the DNA template.bApplication of the DNA-to-RNA method...
We have developed a highly efficient, template- and purification-free, adapter adenylation method using T4 RNA ligase 1. This method is capable of adenylating large amounts of adapter at ~100% efficiency and can efficiently adenylate both DNA and RNA bases. We find that the adenylation reaction...
摘要: Current methodologies used to synthesize DNA and RNA are reviewed. These focus on using controlled pore glass and microarrays on glass slides.关键词: controlled pore glass DNA nucleic acid chemical synthesis polymer support RNA synthetic nucleic acid ...
MessengerRNA(mRNA) synthesis using DNA as thetemplateis called transcription. The mRNA carriesgeneticinformation from the DNA of the chromosomes in the nucleus to thesurfaceof the ribosomes in the cytosol. It is synthesized as a single strand. Chemically, RNA is similar to DNA. It is an unbr...