1) 质量控制（Quality Control, QC）：使用工具如FastQC检查原始测序数据的质量，评估读段长度、碱基质量...
Integration with other data types 将RNA-seq数据与其他类型的全基因组数据集成,可以将基因表达的调控与分子生理学和功能基因组学的特定方面联系起来。整合分析将RNA-seq数据作为主要基因表达读数与其他基因组实验进行比较,越来越普遍。以下,我们讨论这类分析所面临的一些额外挑战。 一、DNA sequencing DNA序列 RNA和DNA...
序言 “A comprehensive evaluation of normalization methods for Illumina high-throughput RNA sequencing data analysis”发表于2013 的《Briefings in Bioinformatics 》 杂志上,这篇文献对TC,UQ,Med,DESeq,TMM,Q,RPKM等七种的数据标准化方法进行了综合评估,最终得出了结论--这七种方法中DESeq 和 TMM两种数据标准...
1.RNA sequencing: the teenage years 2.A survey of best practices for RNA-seq dataanalysis 3.Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud The End 怀念那些无所事事一整个夏天的年少
RNA-Seq Data Analysis: A Basic Workflow RNA-Sequencing (RNA-Seq) has revolutionized the field of genomics, providing unprecedented insights into gene expression patterns. The basic workflow for RNA-Seq data analysis involves several crucial steps, each crucial for extracting meaningful information from...
【6】Andrews, T.S., Kiselev, V.Y., McCarthy, D., and Hemberg, M. (2021). Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data. Nat. Protoc. 16. 在学习分析之前,先看看,尤其是标红的文献,了解下分析流程,分析过程中使用的软件,各种软件的优缺点等等背景知识。
近日,湖南大学DrugAI实验室在Cell子刊《Cell Reports Methods》上发表名为”Graph Embedding and Gaussian Mixture Variational Autoencoder Network for End-to-End Analysis of Single-Cell RNA-Sequencing Data”的研究论文,提出了一种用于scRNA-seq的“dropout”插补和特征提取的深度学习框架autoCell。autoCel是一种变分...
题目:bridging data and dynamics in single cells through machine learning 时间: 10月10日星期二 11:00 地点:校本部教二楼 309 reports 摘要 part/2 the rapid development of single-cell sequencing technologies provides unprecedented resolut...
所用的试剂盒为Direct RNA Sequencing Kit (SQK-RNA004),首先准备 poly(A) 富集的mRNA (300ng/8ul) 或者总RNA(1ug/8ul) ,通过 poly(T) 引物反转录合成cDNA链(稳定mRNA),为RNA-cDNA加上测序接头,最后在MinION或PromethION芯片上进行测序(图11),建库总用时大约为2小时15分钟。互补cDNA链不会被测序,只是...
RNAdataanalysis RNAbigdataanalysis RNAsequencing:Transcriptome RNA-seq简述 目录 RNA-seq常规分析RNA-seq高级分析 RNA-seq拓展 RNA-seq简述 RNA-seq简述 WangZ.NatRevGenet,2009.转录组学 ThetranscriptomeisthesetofallRNAmoleculesinonecellorapopulationofcells.ItissometimesusedtorefertoallRNAs,orjustmRNA,dependingon...