3. 下游分析 - Downstream analysis 下游分析主要是通过将可以解释的模型拟合到数据中获得相应的结论,比如有相似基因表达谱的细胞群代表一个细胞簇、相似细胞之间基因表达的微小变化指示连续(分化)轨迹;或具有相关表达趋势的基因指示共调控等。 下游分析可分为细胞水平和基因水平的方法,如图所示。细胞水平分析通常着重于两...
RNA-Sequencinganalysis罗氏454FLX+illuminaHiSeq2000二代测序仪测序芯片罗氏454illuminaDNA(0.1-1.0ug)SinglemoleculearraySamplepreparationClustergrowth5’5’3’GTCAGTCAGTCACAGTCATCACCTAGCGTAGT123789456ImageacquisitionBasecallingTGCTACGAT…SequencingAndreasGogol-Do¨ringandWeiChenRNA-seqcanbeusedtodeterminetheexpression...
Our analysis also revealed that the highest representation of regulons defined the unspecialized group of neurons. Because the enrichment and number of effector genes, together with the expression level of the master TF, are key parameters in predicting the activity of a regulon, a potential limitat...
额外学习:Clustering Validation Statistics: 4 Vital Things Everyone Should Know - Unsupervised Machine Learning Comparisons of trajectory analysis methods. 这部分的数据是如何模拟的,必须要知道。依然不能真正模拟真实的情况,要控制表达基因的数量,同时mix不一定是线性的,这点文章也承认了。 评估的工具list: Slings...
this is achieved by using the Seq2Fun algorithm to map reads to a comprehensive ortholog database – EcoOmicsDB (described below). While the primary motivation for ExpressAnalyst is to improve RNA-seq analysis for non-model organisms, it can also be used to analyze data from common model or...
Sequence Analysis, RNARNA SplicingAlgorithmsModels, GeneticSoftwareMOTIVATION: A new protocol for sequencing the messenger RNA in a cell, known as RNA-Seq,... C Trapnell,L Pachter,SL Salzberg - 《Bioinformatics》 被引量: 1.4万发表: 2009年 Next Generation RNA Sequencing of Acute Promyelocytic Leu...
RNA Sequencing Analysis of the Gametophyte Transcriptome from the Liverwort, Marchantia polymorpha 来自 NCBI 喜欢 0 阅读量: 57 作者:N Sharma,CH Jung,PL Bhalla,MB Singh 摘要: The liverwort Marchantia polymorpha is a member of the most basal lineage of land plants (embryophytes) and likely ...
title:Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis journal:cell stem cell IF: 21.46 摘要:精子发生产生成熟的雄性配子,对遗传信息的跨代的稳定传递时至关重要的。然而,人类生精过程的发育上的宏观特征目前还是未知的。我们使用了单细胞RNAseq的技术检测了...
For RNA-Seq data analysis, we developed a statistical method for the estimation of isoform-specific gene expression with massively parallel RNA sequencing. We count the number of reads falling into each exonic region of a gene and use a joint Poisson model to study the joint distribution of ...
which use non-equilibrium dynamical systems theory to analyze the stability of attractors within data and identify transition-driving genes in gene expression and splicing processes. mathematical theory based on the popular splicing analysi...