Software & Analysis Services Popular Products Explore All Products Instruments Sequencing Platforms Microarray Scanners IVD Instruments All Instruments For every lab, everywhere Access NGS with unrivaled simplicity and unthinkable speed Meet the MiSeq i100 Series Kits & Reagents Library Preparatio...
47335812 + 0 in total (QC-passed reads + QC-failed reads) 3734708 + 0 secondary 0 + 0 supplementary 0 + 0 duplicates 46714923 + 0 mapped (98.69% : N/A) 43601104 + 0 paired in sequencing 21800552 + 0 read1 21800552 + 0 read2 42216752 + 0 properly paired (96.82% : N/A) 4287978...
在今天的案例中,我们以 PBMC数据集为例。 首先,打开JMP Genomics 10菜单Import>Next-Gen Sequencing,以稀疏格式读取原始UMI计数数据,然后将其转换为密集格式。 然后,可以将输出的密集数据用作基本单细胞RNA-seq工作流程的输入数据,此工作流程位于Workflows>Basic子菜单。在界面中指定设置后运行后,可以在JMP Project里生...
raw_feature_bc_matrix.h5 analysis文件夹下有如下结果: clustering/ diffexp/ pca/ tsne/ umap/ 到此,就可以得到很多单细胞测序的分析结果了,结果解读,下期见~ 附:Bcl2fastq 的安装 下载路径: https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software/downloads.html 下载版本:...
一、DNA sequencing DNA序列 RNA和DNA测序的结合可以用于多种目的，例如单核苷酸多态性（SNP）的发现、...
Software & Analysis Services Popular Products Explore All Products Instruments Sequencing Platforms Microarray Scanners IVD Instruments All Instruments For every lab, everywhere Access NGS with unrivaled simplicity and unthinkable speed Meet the MiSeq i100 Series Kits & Reagents Library Preparatio...
runs faster on all data sets tested to date compared with other assembly software, including Cufflinks. Ballgown bridges the gap between transcriptome assembly and expression analysis To the Editor: Analysis of raw reads from RNA sequencing (RNA-seq) makes it possible to reconstruct complete gene ...
Here we present a comprehensive, temporal program of gene expression data encompassing human PC differentiation (PCD) using RNA sequencing (RNA-seq). Our results reveal 6374 differentially expressed genes classified into four temporal gene expression patterns. A stringent pathway enrichment analysis of ...
@Article{,author={Matthew E Ritchie and Belinda Phipson and Di Wu and Yifang Hu and Charity W Law and Wei Shi and Gordon K Smyth},title={{limma} powers differential expression analyses for {RNA}-sequencing and microarray studies},journal={Nucleic Acids Re...
43601104 + 0 paired in sequencing 21800552 + 0 read1 21800552 + 0 read2 42216752 + 0 properly paired (96.82% : N/A) 42879780 + 0 with itself and mate mapped 100435 + 0 singletons (0.23% : N/A) 337412 + 0 with mate mapped to a different chr ...