In addition to the use of Cas9 for enhancer deletion, variant forms of Cas9 can be used for enhancer activation or repression. Dead Cas9 (dCas9), which is generated from nuclease-activated Cas9, can be fused to activation effectors (such as VP64 and p65 proteins) or inhibition effectors (s...
The double-strand specific nuclease (DSN) method was developed to also detect short overhangs of minimally 12 nucleotides in length (Zhao et al. 2008). This approach has been successfully used for human cell lines and provides a way to specifically assess the impact of telomerase dysfunction on...
Rockville, MD: US Dept of Health and Human Services, Public Health Service, Agency for Health Care Policy and Research; 1995. 156. Rathore SS, Hinn AR, Cooper LS, Tyroler HA, Rosamond WD. Characterization of incident stroke signs and symptoms: findings from the atherosclerosis risk in ...
their levels of oral Spanish skill and scored much higher than a comparison group of non-treated. A subset of graduates of the two-year program was tested as kindergarteners; they showed a continued advantage over a comparison group of 18 peers who had not received the intervention. For the...
As a human replacement, the crab-eating macaque (Macaca fascicularis) is an invaluable non-human primate model for biomedical research, but the lack of genetic information on this primate has represented a significant obstacle for its broader use. Here,
Universal Health Coverage is widely endorsed as the pivotal goal in global health, however substantial barriers to accessing health services for children in low and middle-income countries (LMIC) exist. Failure to access healthcare is an important contri
ATMis frequently implicated in hereditary BC as a low-penetrance susceptibility gene. TheATMkinase has an essential role maintaining genomic integrity, as a key activator of cellular responses to DNA double-strand breaks [61]. In Chile and Mexico, association studies were performed to evaluate the...
. Positive-strand RNA viruses are a large group of viruses that includes many important human pathogens such as Hepatitis C virus (HCV), SARS, SARS-CoV-2, HIV, etc. and share a high degree of similarity in their replication cycles. Therefore, understanding strategies for replication may go ...
In the study by Herrel [24], the authors also felt that there was an urgent need for the healthcare staff to understand the cultural differences of Somali women. This study also suggested that all members of a healthcare team should receive cultural sensitivity training that includes topics ...
Immunoglobulin light chain and heavy chain first strand cDNAs can be prepared from mRNA derived from lymphocytes of a subject immunized with a protein of interest using primers specific for a constant region of the heavy chain and the constant region of each of the κ and γ light chains. ...