荧光素酶报告系统 luciferase reporter assay design
BasicReporterAssayPrinciple TREATMENT REPORTER ASSAY REPORTERONSTRUCT nio i ssrepExld l o F Figure1 PromegaCorporationromegaCorporation5©2014PromegaCorporation TranscriptionalFusiontotheReporterGene REPORTERromoter ASSAYrResponseElementusiontranscriptionEPORTERONSTRUCT ...
Basic Reporter Assay Principle REPORTER n o TREATMENT i ASSAY s s e r p x E d l o F Figure 1 REPORTER CONSTRUCT Promega CorporationPromega Corporation 5 ©2014 Promega Corporation. Transcriptional Fusion to the Reporter Gene Promoter REPORTER n o or i ASSAY s s e Response Element r p ...
Here we describe a rapid, sensitive and simple reporter-based assay for the validation of genes putatively associated with A尾-mediated neurotoxicity, which can in principle be extended to the validation of targets in the context of other neuronal insults. The assay is suitable for the generation...
Principle The Luciferase Reporter Gene Assay contains all substrate components for the enzymatic determination of firefly luciferase activity, as well as a lysis buffer for the mild and rapid extraction of luciferase from eukaryotic cells. In the first step, transfected cells are lysed by the addition...
(ii) the use of mCherry autofluorescence allows a low cost and easily performed cell-based biosensor assay without any chromogenic reagent; and (iii) the use of HCS measurement of fluores- cence in microplates allows visualization, automatic operation, real-time monitoring and high-throughput data...
As a proof-of-principle, they used an antiviral serum collected from convalescent mice previously infected with PR8. IAV-Gluc was pre-incubated with serial dilutions of the antiviral serum, the serum-virus mixture was used to infect MDCK cells in 96-well plates, and the biol...
Interestingly, regions that are strongly bound by TFs and cofactors, or bound by multiple TFs are more likely to be active in a transgenic reporter assay [32], [52]. A more widely used method for genome-wide annotations of enhancers is based on mapping of enhancer-associated H3K4me1 and ...
Additional file 11: Figure S6: Responses to septic plasma measured in a PMN reporter assay using PMNs from an additional healthy donor. Results from experiment III as shown in Figure 4B are replicated here in (A). PMNs from an additional donor were treated with the same set of plasma sample...
Bacterial Luciferase Assay Principle The bacterial gene cassette (lux) encodes six components that are necessary for transfected cells to luminesce. The heterodimeric bacterial luciferase is encoded by luxA and luxB genes. In addition, a reductase, a transferase, and a synthase are encoded by luxC,...