The invention relates to methods and compositions for assessing protein stability, including improved assays for distinguishing between soluble and aggregated proteins. The methods and compositions include meas
Protein stability is particularly important for the accuracy of the MbYTH assay. Degradation of the bait protein may release the Cub-LexA-VP16 module into the nucleus and induce the expression of the reporter genes. Degradation of the prey protein may reduce its “critical mass” level needed ...
Protein Assay Data Analysis Protein assay technical guide –tools and reagents for improved quantitation of total or specific proteins. Protein assay compatibility table –summarizes compatible substances for several popular protein assays. Protein Analysis Learning Center –resources for different protein anal...
Reagents, vectors, and kits for expressing and detecting a variety of reporter genes. Enzyme and Protein Activity Assays Reagents and assays for detecting enzyme activity by absorbance, fluorescence, or chemiluminescence Pro-Detect Rapid Assays Single use, dipstick-style lateral flow assay strips for ...
hypothesized that SULI should have high tolerance to fusion at different positions of the target protein. To test this hypothesis, we fused SULI to the N-terminus, C-terminus or inner position of the reporter protein (Fig.2a, c, e) and tested its ability to control the stability of the ...
Potel, C. M. et al. Impact of phosphorylation on thermal stability of proteins.Nat. Methodshttps://doi.org/10.1038/s41592-021-01177-5(2021). Gaetani, M. et al. Proteome Integral Solubility Alteration: a high-throughput proteomics assay for target deconvolution.J. Proteome Res.18, 4027–40...
Despite the caveat of PCA that interaction kinetics and dynamics are compromised due to the stability of the reconstituted PCA protein, its widespread applicability and flexibility, coupled with the continuous development of new strategies and reporter systems, ensure that this system will continu...
this stability was established although unbound, competition–competent Gal4 molecules were present in excess amounts. During immobilization on its promoter, expression of the reporter gene occurred indicating that Gal4 functions via long-lived complexes with promoters during transcriptional induction (Nalley...
The stability of the cultivations was monitored with online and off-line measurements, including a monitoring for stability of transcription of the 31 reporter genes covering essential cellular processes [66]. We used the strain Rut-C30, instead of the sequenced QM6a strain, due to its improved...
Protein expression and lysosomal stability were observed in Mytilus edulis exposed to 0, 20, 40, 60, and 80 ppb copper sulfate for 24 hours in a static exposure system. Haemolymph was removed from each mussel and analyzed for lysosomal damage using the Neutral Red retention assay expressed as...