assayNuclear receptors act as ligand-activated transcription factors translating ligand signals into changes in gene expression. The 48 members of the superfamily of nuclear receptors identified so far are involved amongst others in maintenance of metabolic balance, inflammation, and cancer. Thus, they ...
A reporter gene encodes a protein that is expressed when certain conditions are met. Reporter genes can signify the entire construct as well as the protein to be detected; in the case of fusion proteins, only a part of the protein demonstrates the activity to be detected. In principle, every...
Principle The Luciferase Reporter Gene Assay contains all substrate components for the enzymatic determination of firefly luciferase activity, as well as a lysis buffer for the mild and rapid extraction of luciferase from eukaryotic cells. In the first step, transfected cells are lysed by the addition...
In this study, a reporter gene assay based on the HEK293 cell line was established that expresses LHCGR and a luciferase reporter under the control of cAMP responsive element binding protein. As shown in Fig. 1, the basic principle is that LHCGR belongs to the G protein-coupled receptor fami...
Properties of reporter genes must be considered on a per experiment basis because different genes will serve different purposes and choosing the best reporter gene assay depends on the type of study (Table 1) [39]. For example to observe localization, fluorescent genes like GFP ...
荧光素酶报告系统 luciferase reporter assay design
The rpoS level has been monitored by the aim of a transcriptional reporter gene based on the synthesis of the green fluorescent protein (GFP). It has been observed that the level of GFP increases during the transition from batch to fed-batch phase. After this initial increase, the GFP conten...
We sought to establish and evaluate a reporter gene system using TYR under the control of the Tet-on gene expression system (gene expression induced by doxycycline [Dox]) as a multimodality imaging agent. We transfected TYR into human breast cancer cells (MDA-MB-231), naming the resulting ...
correspondEnce A coincidence reporter-gene system for high-throughput screening signals mediated by the known FLuc and RLuc stabilizers, PTC124 and BTS, respectively (Fig. 1b,c and Supplementary Fig. 2). Using the LOPAC1280 chemical library, we conducted a quantitative HTS experiment, in which ...
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