Reporter gene assayCytotoxicityFireflyRenillaThe activity of nuclear receptors (e.g., pregnane x receptor, PXR) can be assessed by luminescence-based dual reporter gene assays. Under most conditions, receptor-activated firefly luminescence is normalized to Renilla luminescence, which is triggered by a ...
Ask TaqMan #34 Copy Number Variation – How Does It Work? microRNA Quantification using TaqMan® Advanced miRNA Assays requires a cDNA synthesis step followed by the detection using real-time PCR. The TaqMan Advanced miRNA Assay workflow. ...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
Instead of using intercalating dyes, this assay uses TaqMan probes with a 5' fluorescent reporter dye and a 3' quencher dye. These probes are target-specific, and only bind to the DNA sequence of interest downstream of one of the primers during the annealing step. When the enzyme Taq polym...
For the estimation of PCR efficiency the standard used to construct the standard curve does not have to be calibrated. The efficiency (E) of PCR is defined as the fraction of target molecules that are copied in one PCR cycle [10], [11]. A properly designed assay shall, in the absence ...
The expression of a full-length 'sense' version of messenger RNA for this gene leads to the production of Md1, and to male sex determination (Fig. 1). The expression of an RNA that does not produce protein and is tran- scribed from the opposite strand of DNA at the md1 locus — ...
However, parafilm does not rupture uniformly. Sometimes the gas pulse blows a nice hole through the center and disperses the tungsten particles well. Other times, it rips off from one side and forms a flap such that most of the particles are not dispersed to the target. The bottom line ...
During vegetative growth, σWhiG is present in Streptomyces cells, yet the expression of its target genes does not increase until later in development. This suggested σWhiG is post-translationally regulated. It was initially unclear how σWhiG activity is regulated, until a bacterial two-hybrid ge...
Taken together, although we noticed extensive actin-mediated ruffling at the plasma membrane and sometimes closure of vesicles, macropinocytosis does not appear to have an important role in DENV cell entry in macrophages. Figure 4 Role of antibody-induced macropinocytosis in virus internalization. (A)...
After the apo-dCas9 assay, both DNA microarrays were de-hybridised to strip off all apo-dCas9 proteins and to generate a ssDNA array out of the dsDNA microarray copy. Our thrombin SCORE experiment was performed in three steps. First, thrombin was flushed over the DNA microarray, followed ...