reporter gene assay (RGA)promoterluciferin.The luminescent reporter gene assay (LRGA) is arguably the most prominent type of reporter gene assay used in biomolecular and pharmaceutical development laboratories. Part of this popularity is due to the high signal associated with luciferases, the ...
Define Reporter gene assay. Reporter gene assay synonyms, Reporter gene assay pronunciation, Reporter gene assay translation, English dictionary definition of Reporter gene assay. n a gene with an easily recognizable phenotype, used in analysing the regu
In the present study, we employed low-background lentivirus carrying sis-inducible element (SIE)-driven firefly luciferase to generate a stable DB-SIE-Luc cell line that expresses endogenous IL-23 receptors and developed a sensitive and straightforward reporter gene assay (RGA) based on DB-SIE-...
FSH-CTP, FSH-C-terminus part; PTH, parathyroid hormone; RGA, reporter gene assay; TSH, thyroid stimulating hormone; LH, luteinizing hormone; hCG, human chorionic gonadotropin. 3.3.2. Accuracy and linearity The accuracy of the RGA was performed by recovery, which is obtained by calculating the...
In this study, we report the development of a robust and reliable cell-based reporter gene assay (RGA) for measuring the ADCC activity of human anti-MRES-spike antibodies. ADCC activity was measured using target 293T/MERS cells that stably express MERS spike protein on their surface and effect...
In addition, stronger, exogenous promoters were tested in replacement of Sp2 in the hope of enhancing cargo gene expression, but although recombinant viruses infectious for PHH were produced, enhanced expression was not demonstrated using infection assay. Similar design was used by ...
Here, we describe the development and validation of a reporter gene assay (RGA) to measure the bioactivity of anti㎜AGmAbs. We established the bioassay based on parental Raji cells and a Jurkat cell line stably transfected with human LAGgene and luciferase reporter elements controlled by nuclear ...
A reporter gene assay (RGA) that uses a mouse liver recombinant Hepalclc7 containing the firefly luciferase gene was developed to screen for dioxins in human plasma. For a high-sensitivity method, the addition of cycloheximide to the culture medium brought about a fivefold increase in the ...
(RGA)The luminescent reporter gene assay (LRGA) is arguably the most prominent type of reporter gene assay used in biomolecular and pharmaceutical development laboratories. Part of this popularity is due to the high signal associated with luciferases, the foundation of this technology. This feature...
In the present study, we developed reporter gene assay (RGA) systems to detect molting hormone (ecdysone) activity by introducing EcR–USP cDNAand a bacteriallacZreporter gene into yeast. EcR andUSPwere derived from the insect species of three different taxonomic orders:Drosophila melanogaster(...