4. Uehata, T., et al., Malt1-induced cleavage of regnase-1 in CD4(+) helper T cells regulates immune activation.Cell,2013. 153(5): p. 1036-1049.
将肿瘤特异性的T细胞输送到病人体内进行治疗的方法叫做适应性T细胞疗法(adoptive T-cell therapy,ACT)。ACT通常需要从病人血液或肿瘤中分离出T细胞,然后这些细胞在实验室中被刺激,增加它们的数量或者对它们进行改造增强它们的功能,然后回输给病人【2】。例如,使T细胞表达肿瘤特异性的嵌合抗原受体(chimaeric antigen recepto...
Regnase-1 directly targets Tcf7 mRNA; its deficiency augments TCF-1 expression leading to the formation of TPEX that support long-term CAR-T cell persistence and function. Regnase-1 deficiency also reduces exhaustion and enhances the activity of TCF-1- CAR-T cells. We further validate these ...
Activated T cells often become less functional in the tumor microenvironment or during chronic infection. In Nature, Chi and colleagues describe an unbiased CRISPR-Cas9 screen in CD8+ T cells that identifies Regnase-1 as a negative regulator of T cell function within the tumor environment. Regnase...
[5]Mino, T. et al. Regnase-1 and Roquin regulate a common element in infammatory mRNAs by spatiotemporally distinct mechanisms. Cell 161, 1058–1073 (2015). [6]Fu, M. & Blackshear, P. J. RNA-binding proteins ...
Cell Reports; IF:8.800; DOI: 10.1016/j.celrep.2023.113392 内容概要: ① 利用RNA-seq和蛋白质组学,鉴定了选择性富集在脑内皮细胞(endothelial cells,ECs)中的转录本和蛋白质,并证明了它们的部分相关性。 ② 利用scRNA-seq,解析了脑ECs功能异质性的分子基础,并通过整合表观基因组学和转录组学,确定了TCF/LEF...
REGNASE-1 lossreprogramsTcellsin TME Toaddresscell-intrinsice,,ectsmediatedbyREGNASE-1(weper)ormed RNAsequencing(RNA-seq)o,REGNASE-1-nullandwild-typeOT-Icells isolated,romtheinvivodualtrans,ersystem.Genesetenrichmentanal-ysis(GSEA)usinggenemodulesassociatedwith di,(erent)unctional stateso,tumour-in...
Cell Reports; IF:8.800; DOI: 10.1016/j.celrep.2023.113392 内容概要: ① 利用RNA-seq和蛋白质组学,鉴定了选择性富集在脑内皮细胞(endothelial cells,ECs)中的转录本和蛋白质,并证明了它们的部分相关性。 ② 利用scRNA-seq,解析了脑ECs功能异质性的分子基础,并通过整合表观基因组学和转录组学,确定了TCF/LEF...
Regnase-1 is also essential for preventing aberrant effector CD4 + T cell generation, by limiting the expression of a set of genes, including Ox40, IL-2, and c-Rel in T cells [2]. T cells deficient of Regnase-1 were spontaneously activated and exhibited effector/memory phenotype. ...
将细胞上清与SH-SY5Y细胞(神经母细胞瘤细胞株)共孵育8,12或24小时后,采用CCK-8试剂盒(Cell Counting Kit,CCK-8)检测SH-SY5Y细胞的细胞活性,并在光学显微镜下观察细胞状态;采用AnnexinⅤ-FITC/PI染色后在流式细胞仪上检测SH-SY5Y细胞的死亡率。 6.统计分析 本研究全部数据均采用SPSS20.0统计分析软件进行分析,两...