Dynamic changes in gene expression are key factors in the development and activation of immune cells. RNA metabolism is one of the critical steps for the control of gene expression. Together with transcriptional regulation, mRNA decay by specific ribonucleases (RNases) plays a vital role in ...
2、空间转录组学揭示睡眠不足导致的不同脑区独特的基因表达变化 Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation Nature Communications; IF:16.600; DOI: 10.1038/s41467-023-42751-z 内容概要: ① 利用空间转录组学,研究了短暂睡眠不足对雄性小鼠整...
Thus, we next investigated whether these chemokine gene signatures were dependent on Regnase-1 expression by analyzing the expression of chemokines in skin specimens from wild-type, Regnase-1+/-, and Regnase-1-/- mice (Fig. 4). The protein levels of CCL3, CCL4, CCL5, CCL7, CCL8, CCL...
Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation Nature Communications; IF:16.600; DOI: 10.1038/s41467-023-42751-z 内容概要: ① 利用空间转录组学,研究了短暂睡眠不足对雄性小鼠整个大脑的影响,发现睡眠不足会导致整个大脑的基因表达出现明显差异,...
Regnase-1 acts like a pair of scissors, chopping up inflammation-associatedgene productsso they can't be expressed. By targeting genes turned on in response to inflammation, Regnase-1 prevents the body's immune system from going haywire. However, through a process called phosphorylation, Regnase-...
(B) Scatter plot of gene expressions in naive and effector CD4 T cells from Mx-Cre+Regnase-1+/+ or Mx-Cre+Regnase-1fl/fl mice. The mice were treated with Poly I:C as in (A). Cells were sorted by FACS and total RNA was subjected to microarray analysis using Affymetrix mouse Genome...
Background The application of CRISPR/Cas9-gene-editing to enhance the anti-tumor activity of T cell Adoptive Cell Therapies (ACT) is a promising approach in the treatment of patients with solid tumors. We developed an in vivo CRISPR^2 screening approach and interrogated the top dual-edit combin...
iltratingREGNASE-1-nullandwild-typecells.Moti,searchesonaccessibleregionsidenti,iedanenrichmento,TCF-1,BACH2andBCL6—butdownregulated IRF4moti,s—inREGNASE-1-null cells(ExtendedDataFig.2g,h).Thus,REGNASE-1-nullCD8+Tcellsare reprogrammedintheTMEwithenhancedgene-expressionprograms associatedwithnaiveor...
Here, we provide a comprehensive update on the function, regulation and molecular mechanisms of Regnase-1, and we propose that Regnase-1 may function as a master rapid response gene for cellular adaption triggered by microenvironmental changes....
states including autoimmune diseases,cancer and cardiovascular diseases.Here,we provide a comprehensive update on the function,regulation and molecular mechanisms of Regnase-1,and we propose that Regnase-1 may function as a master rapid response gene for cellular adaption triggered by microenvironmental ...