2. Lim, W.A. and C.H. June, The Principles of Engineering Immune Cells to Treat Cancer.Cell, 2017. 168(4): p. 724-740. 3. Henning, A.N., R. Roychoudhuri, and N.P. Restifo, Epigenetic control of CD8(+) ...
将肿瘤特异性的T细胞输送到病人体内进行治疗的方法叫做适应性T细胞疗法(adoptive T-cell therapy,ACT)。ACT通常需要从病人血液或肿瘤中分离出T细胞,然后这些细胞在实验室中被刺激,增加它们的数量或者对它们进行改造增强它们的功能,然后回输给病人【2】。例如,使T细胞表达肿瘤特异性的嵌合抗原受体(chimaeric antigen recepto...
We have constructed mice with a single point mutation at the catalytic center of the Regnase-1 RNase domain, which lacks endonuclease activity. D141N mutant mice demonstrated systemic inflammation, immune cell infiltration into various organs, and progressive development of lung...
[2]Mino, T. et al. Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade infammatory mRNAs. Nucleic Acids Res. 47, 8838–8859 (2019). [3]Uehata, T. et al. Malt1-induced cleavage ...
Furthermore, the impairment of Regnase-1 results in excessive and sustained inflammation that leads to inflammatory diseases, including T-cell-specific autoimmune diseases [2]. Regnase-1 activity has been reported to be associated with the pathology of psoriasis, a chronic autoimmune disease leading ...
REGNASE-1-de,cientCD8+Tcells.Bycontrast,thetargetingo,additionalsignalling,actors—includingPTPN2andSOCS1—improvesthetherapeuticefcacyo,REGNASE-1-de,cientCD8+Tcells.Our,ndingssuggestthatTcellpersistenceande(ector)unctioncanbecoordinatedintumourimmunityandpointtoavenues,orimprovingtheefcacyo,adoptivecell...
Moreover, in T cells, Regnase-1 regulates the mRNAs of a set of genes, including c-Rel, Ox40, and Il2, through cleavage of their 3′ UTRs. Interestingly, T cell receptor (TCR) stimulation leads to cleavage of Regnase-1 at R111 by Malt1/paracaspase, freeing T cells from Regnase-1-...
2023年3月15日,宾夕法尼亚大学Carl June团队在《美国国家科学院院刊》(PNAS)上发表了题为:Combined disruption of T cell inflammatory regulators Regnase-1 and Roquin-1 enhances antitumor activity of engineered human T cells的研究论文。 该研究开发了一种帮助T细胞攻击实体瘤的“组合拳”新方法,通过CRISPR-...
The regnase-1 knockdown human T lymphocyte leukemia cell (Jurkat T) model was constructed by small interfering RNA (siRNA) technology. Then PCR and Western blot were used to detect the transcription level and protein level of downstream genes. Co-immunoprecipitation was used to verify the ...
Regnase-1 (also known as Zc3h12a and MCPIP1) is an RNase that destabilizes a set of mRNAs, including Il6 and Il12b, through cleavage of their 3′ UTRs. Although Regnase-1 inactivation leads to development of an autoimmune disease characterized by T cell activation and hyperimmunoglobulinemia...