Quantitative real-time PCR (qRT-PCR) has becoming a widely used method for detection and characterization of gene expression in recent years. However, the ... Y Li,W Chen,Q Wang,... - 《Australasian Plant Pathology》 被引量: 1发表: 2014年 Selection and validation of reference genes for ...
Quantitative real-time PCR (qRT-PCR) has become widely used method for detecting gene expression of an organism at different developmental stages or under various stressful environmental conditions. Finding reference genes is a prerequisite in this process. In this research, we investigated the ...
Stability analysis of gene expression followed by pairwise variation (Vn/Vn + 1) analysis revealed that PGK1, HSP90AA1 and GYPC were the most stable reference genes and suitable for qRT-PCR normalisation in both HLN and liver tissues. These three genes were validated against FoxP3, IL-...
a我的问题是我们可以去book I am the problem is that we can go to book;[translate] aValidation of candidate bovine reference genes for use with real-timePCR 候选人迟钝的参考基因的检验为使用与真正timePCR[translate]
Quantitative real-time PCR was used to measure the RNA transcription level of various housekeeping genes in 16 different human tissues: heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leu...
1.The selection of reference genes in young mouse small intestine幼龄小鼠小肠组织内参基因的选择 2.The selection of reference genes plays a key role in the study of gene expression by real-time quantitative PCR.本文用GeNorm与NormFinder算法分析actin、α-tubulin、cblp和yptc1四个常用内参基因在不同处...
Reference genes are frequently used as normalizers for expression studies despite not being previously verified to present suitable stabilities. Considering the interest that tiger beetles have generated in the past years, resulting in a variety of studies, it is crucial to dispose of a validated ...
Quantitative real-time PCR (RT-qPCR) is widely used to analyze gene expression. To minimize the impact of variances from RT-qPCR, a rigorous selection of reference genes must be performed prior to any experiments due to variations in experimental conditions. In this study, CD34+ mononuclear ...
SHORT-COMMUNICATION Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum) Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new ...
Figure 1: Expression profiles of candidate reference genes inN. lugens. The expression level of candidate references in all tested samples are documented in Ct-value, and showed as boxplot. The dot indicates the maximum or minimum value of Ct, while whiskers indicate the standard error (SE) of...