Morley AA. Digital PCR: A brief history. Biomol Detect Quantif 2014;1(1):1-2 doi: 10.1016/j.bdq.2014.06.001 Taylor SC, Laperriere G, Germain H. Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Scien...
Reference gene normalisation is recommended for gene expression analysis using alternative dPCR approaches, thereby circumventing any impact from RNA sample contaminants1,22. Using experimentally generated mixtures of RNA and dPCR assays for proteinase 3 (PRTN3) expression, our results show that normalisa...
1.Taylor, S.C., Laperriere, G. & Germain, H. Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Sci Rep 7, 2409 (2017). https://doi.org/10.1038/s41598-017...
• Sanchez-Freire, Ebert, A.D., Kalisky, T., Quake, S.R., Wu, J.C. April 5, 2012. Microfluidic single-cell real-time PCR for comparative analysis of gene expression traits. Nature Protocols • Luc, S., Jacobsen, S.E.W., et al. February 19, 2012. The earliest thymic T cel...
The most susceptible, frustrating and often most interesting samples are those containing low abundant targets with small expression differences of 2-fold or lower. Here, Droplet Digital PCR (ddPCR) and qPCR platforms were directly compared for gene expression analysis using low amounts of purified,...
Polymerase Chain Reaction Amplification (PCR) is one of the most actively used techniques in molecular biology. In recent years, PCR has been increasingly used for gene expression detection or quantification. It is a more convenient method in gene expression studies comparing to other techniques, suc...
A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res 18, 2887–2890. Stachel SE, Timmerman B, Zambryski P (1987). Activation of Agrobacterium tumefaciens vir gene expression gener- ates multiple single-stranded T-strand ...
2.The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments[J]. Clinical Chemistry.2009. January; 55:4 611–622 3.Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 22DDCT Method[J].. METHODS .2001; 25, 402–408. ...
图3.9 一篇文章中对于板间校正的设置(文章标题:Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus) 2)简化版方式:根据板间校正的原理,主要控制的是各板之间的内参基因,因此:如果使用的qPCR体系完全一致(包括样本、酶、qPCR板子/膜、仪器等),...
Protocols for PCR may vary depending upon the applications. This video provides an overview on routine PCR, hot-start PCR, high-fidelity PCR, GC-rich PCR, and long PCR. Gene expression Variations in gene expression among cell types, tissues...