Stability analysis of gene expression followed by pairwise variation (Vn/Vn + 1) analysis revealed that PGK1, HSP90AA1 and GYPC were the most stable reference genes and suitable for qRT-PCR normalisation in both HLN and liver tissues. These three genes were validated against FoxP3, IL-...
Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum)Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new t...
Reference genes are frequently used as normalizers for expression studies despite not being previously verified to present suitable stabilities. Considering the interest that tiger beetles have generated in the past years, resulting in a variety of studies, it is crucial to dispose of a validated ...
Quantitative real-time PCR (qRT-PCR) is a powerful and sensitive methodology to analyze expression of target genes. qRT-PCRs rely on selecting suitable reference gene. In the present study, 17 candidate reference genes in Hainan medaka, Oryzias curvinotus, were selected, and candidate genes were...
A critical step in the RT-qPCR workflow for studying gene expression is data normalization, one of the strategies being the use of reference genes. This study aimed to identify and validate a selection of reference genes for relative quantification in Ta
Real-time rtPCR was used to establish RNA expression levels. Data analysis was carried out using geNORM. Results When using HaCaT or adult NHEK cells any combination of 8 of the housekeeping genes would be appropriate for normalisation. In contrast, with juvenile NHEK cells only 4 of the ...
Several tech- niques have been developed to measure expression levels, among which the coupling between reverse tran- scription and quantitative (real-time) PCR (RT-qPCR) appears to be the most appropriate to study limited numbers of genes in large sets of conditions [18,19]. Significant ...
A critical step in the RT-qPCR workflow for studying gene expression is data normalization, one of the strategies being the use of reference genes. This study aimed to identify and validate a selection of reference genes for relative quantification in Ta
SHORT-COMMUNICATION Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum) Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new ...
Marcos Fernando Basso1, Bárbara Andrade Dias Brito da Cunha1, Adilson Kenji Kobayashi1 & Hugo Bruno Correa Molinari1 Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but reliable results depend on the use of stable reference genes for ...