Secretin partial unbinding and binding protocol The secretin:SecR complex (obtained removing both the G protein and Nb35) was prepared and equilibrated as reported above. The secretin partial unbinding was then simulated in two different well-tempered metadynamics replicas68, biasing the distance betwe...
Filtration assayRadioligand-receptor bindingSR141716Type-1 cannabinoid receptor (CB1), one of the main targets of endocannabinoids, plays a key role in several pathophysiological conditions that affect both central nervous system and peripheral tissues. Today, its biochemical identification and ...
In order to access the FRBI impacts on the secretion of estradiol (E2), the serum E2 concentrations were determined using the especial ELISA kit for mice (Cusabio Biotech Co., Ltd. Wuhan, P.R. China) according to the kit protocol. Analytical sensitivities were 0.02 pg/mL for E2. All...
Pharmacology EvaluationUsing instructions provided by the assay protocol for Kd determination, competition assays (Ki, IC50), and order of reagent, control, and compound addition to the plate, pharmacology data was generated to validate a fully automated assay procedure illustrated by Figures 5 and 6...
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. 发表在 试剂耗材 | 标签为 anti、Rabbit、receptor、sflt、vegf 阿普洛尔盐酸盐 发表于2024年12月29日由上海金畔生物科技有限公司 阿普洛尔盐酸盐 货号: IA3210 品牌: Jinpan 暂无详情...
Point mutations were generated using the InFusion HD Site-Directed Mutagenesis protocol (Clontech). In all cases, the target proteins were secreted as N-terminal protein-A fusion proteins with a Tobacco Etch Virus (TEV) protease cleavage site following the protein-A tag. Harvested media was ...
The Wellcome Reagents Division of Burroughs Wellcome Company has developed a neuroleptic assay kit for potential clinical use (Lader, 1980). The kit differs in a number of ways from the laboratory protocol detailed above. Calf or bovine caudate tissue is used instead of rat striatum. Furthermore...
Aside from the increased time spent exploring the open arm in the EPM, which warrants further exploration, the present findings suggest modifications to the protocol may be required to produce a more pronounced protracted withdrawal syndrome. This could include a longer duration of oxycodone exposure ...
μM resolution. Fluorescence of the shifted band was quantified using ImageStudioLite software. All Kdcalculations were done with DynaFit 4 software53using a previously described competition protocol54. Percent competition was calculated by the formula:...
Twenty-four hours after transfection, cells were treated with 1 μM dexamethasone, and 24 h following treatment firefly and Renilla luciferase activities were read using a Biotek Synergy plate reader and the Dual-Glo Luciferase Assay System (Promega), according to the manufacturer’s protocol....