我们首先要复盘一下文献中提及方法的细节部分,仔细对比,看是否有疏漏。可以查看一些专门关注 protocol 的数据库,比如 Bio-protocol,这里面会详细罗列所有使用的设备、耗材,以及操作步骤。 然后,理性思考一下,同款标签是否适合你的目的蛋白。通过基因工程在目的蛋白末端融合亲和标签,表达后,亲和层析纯化是我们常规获得大量...
The Gene-Trak DNA hybridization assay protocol consists of the following steps: 1. Enrich the food sample in lauryl sulphate tryptose broth. 2. Add enriched sample to test tubes. 3. Add lysis solution to sample tubes and incubate to release rRNA target. 4. Add probe solution and incubate. ...
Scan the array and extract the data as mentioned in the previous small-molecule CSI protocol section (steps 5 and 6). View chapter Explore book Lactose Repressor Protein: Functional Properties and Structure Kathleen ShiveMatthews,Jeffry C.Nichols, inProgress in Nucleic Acid Research and Molecular Bi...
5Agilent High Sensitivity DNA Assay Protocol12 Preparing the Gel-Dye Mix12 Loading the Gel-Dye Mix14 Loading the Marker15 Loading the Ladder and the Samples16 Inserting a Chip in the Agilent 2100 Bioanalyzer17 Starting the Chip Run18 Cleaning Electrodes after a High Sensitivity DNA Chip Run20 ...
Assay for Transposase-Accessible Chromatin using sequencing analysis showed that the binding peaks of PIF4 and CDF2 on YUC8 (ref. 32) were located in an open chromatin region (Fig. 4a). By contrast, the G-box (CACGTG) in the coding region and other DOF-binding sites (AAAAG) located ...
Figure 19. Size selection by magnetic beads.(A) Size distribution of library fragments with respect to their size cutoff. Above the graph is a description of the basic principle of a two-sided size selection protocol. (B) Sche...
(Molecular Dimensions) at 25 °C for 2 h. Thereafter, 1x SelenoMethionine solution and 0.1 mM IPTG were added and the cultivation was continued overnight at 25 °C. The protein was extracted and purified following the same protocol as for the native ORF6, except that 5 mM ...
The lysate Filtration Cartridge is integrated into the DNA Binding Column and combines the steps of clearing the bacterial lysate with binding the DNA directly to the anionexchange resin. The HiPure Filter Column protocol helps reduce time and ef...
Cape Primroses (genus Streptocarpus), are some of these recalcitrant plants and are selected here as material to develop a high quality, high molecular weight DNA extraction protocol for long read genome sequencing. Results We developed a DNA extraction method for PacBio HiFi sequencing for ...
For the DNA-binding assays with the shorter DNA fragment, a 40 bp hypS promoter fragment was amplified using primers HypS_4471_hypS_fwd and HypS_4471_hypS_rev according to the previously published protocol [34]. As negative control, the 154 bp upstream region of MSMEG_5346 encoding a c-...