The current study was an ExWAS for BMI using WES data from 419,668 UK Biobank participants. Although this represents a subset of the exomes previously reported by Akbari et al.10, we were motivated by recent work demonstrating that, in the context of gene-burden analysis11, the various choi...
SRM have been used for theanalysis of proteinsand peptides in body fluids such as blood plasma/serum[154–157]or urine[158,159]. It has been successfully applied to the analysis of Hb variants relevant in hemoglobinopathies[154,155]. Moat et al. developed a protocol for new-born blood ...
In vitro binding assay In vitro binding assays were performed following the RNA Bind-n-Seq protocol72with the following modifications. Recombinant Cp was immobilized on Strep-TactinXT coated magnetic beads (20 pmole protein/μl bead slurry) in buffer w (100 mM Tris-Cl pH 8.0, 150 mM ...
It was shown that odorant binding proteins can be effectively linked to the gold gate surface of an EGOFET device through SAM linkers. Such a protocol can be used for the development of EGOFET immunosensors since antibodies or aptamers can be also bound to the electrode in similar manner. I...
Minimum detection level of 5 µg/ml with the enhanced protocol Adaptable to microplates Less protein-to-protein variation than dye-binding methods BCA Protein Assay Applications: Studying protein:protein interactions Measuring column fractions after affinity chromatography Estimating percent recovery...
Assay Kit, whose assay range in the standard microplate protocol is 100-1500 µg/mL. However, one could dilute it 5-fold in buffer (i.e., 1 part sample plus 4 parts buffer) and then use that diluted sample as the test...
Sensitive—detect down to 5 μg/mL with enhanced protocol No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve How the assay works ...
In summary, CCCTC-binding factor (CTCF) BioVyon Protein A provides a column-based assay method for ChIP and other immunoprecipitation-based CTCF binding (target) sites (CTSs) procedures; the rigid porous structure of BioVyon enables a fast and robust protocol with higher ChIP RNA polymerase II ...
Fig. 3: Validation of the R-DeeP protocol by western blot analysis. The mass spectrometry (MS/MS) data (top panel) were compared to the quantitative analyzes of immunoblots (center panel) obtained with anti-PF3D7_0823200 (a) and anti-Aldolase (b) (bottom panel). ...
For this, single and double oligonucleotide-conjugated anti-CD49e were generated following the optimized conjugation protocol given for anti-Igfr-L1 in Fig. 5, with the difference that a DBCO-SS-NHS ester was used instead of an DBCO-NHS ester linker. Anti-CD49e was detected on the surface...