This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. You can customize the potential PCR amplicon's size range, Tm (melting temperature) for the primers and probes, as well as the organism.
AlleleID® designs real time PCR primers that are optimized for the following chemistries viz. TaqMan®, TaqMan® MGB, molecular beacons and FRET. These real time PCR primers are specific, efficient and compatible with the probe that they work with: Primer Specificity:Primers are designed ...
PRIMER DESIGN METHOD FOR REAL TIME PCRPROBLEM TO BE SOLVED: To provide a primer design method of a real time PCR. SOLUTION: A primer set is retrieved by using an already known primer retrieval algorithm based on the design conditions of a primer, and the retrieved primer set is visually ...
Learn more about digital PCR and its applications › Custom solutions Custom assay design Can’t find what you need from our portfolio of predesigned assays? Use ourTaqMan Custom Design Assay Toolto easily design your own assay based on your target sequence of interest or submit the primer/pro...
Real-Time vs Digital vs Traditional PCR TaqMan vs SYBR Chemistry How TaqMan Assays Work Video Series Real-Time PCR Troubleshooting Tool What is UNG/UDG? Avoiding Contamination Calculating Primer and Probe Concentrations Why TaqMan Assay Design is Important Efficiency of Real-...
Real-Time PCR (qPCR) primer design using free online software Real-time PCR (quantitative PCR or qPCR) has become the preferred method for validating results obtained from assays which measure gene expression profiles... B Thornton,C Basu - 《Biochem Mol Biol Educ》...
qPCR product finder tool Real-time PCR kitsTakara Bio scientists are expert enzymologists who are continually working to improve enzymes and buffers for PCR, real-time qPCR, and RT-PCR. You expect a lot from your real-time qPCR reagents—they need to perform at a high level and provide ...
Applications of Real-Time PCR/qPCR Assays Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis, the detection of genetically modified...
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate ...
Learn more about digital PCR and its applications › Custom solutions Custom assay design Can’t find what you need from our portfolio of predesigned assays? Use ourTaqMan Custom Design Assay Toolto easily design your own assay based on your target sequence of interest or submit the primer/pro...