This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. You can customize the potential PCR amplicon's size range, Tm (melting temperature) for the primers and probes, as well as the organism.
AlleleID® designs real time PCR primers that are optimized for the following chemistries viz. TaqMan®, TaqMan® MGB, molecular beacons and FRET. These real time PCR primers are specific, efficient and compatible with the probe that they work with: Primer Specificity:Primers are designed ...
DNA primersSTATISTICSReal-time quantitative PCR is a technique that can measure the content of the target nucleic acid sequence of interest in a given sample. It is mainly divided into absolute and relative quantitative methods. The relative quantification is mainly used in gene e...
Guidelines for absolute quantitative real-time PCR for microbial determination in in vitro gastrointestinal digestionAkkermansia muciniphilain vitro gastrointestinal digestionmicrobial determinationqPCRCurrently, numerous pieces of evidence have demonstrated that the health gut microbiota contributed to physiological ...
Real-Time PCR Data Analysis What is High Resolution Melting (HRM)? Introduction to qPCR System MIQE and RDML Guidelines qPCR/Real-Time PCR Reagents Real-Time PCR Troubleshooting Oligonucleotides: Design and Applications Introduction to PCR Primer & Probe Chemistries Nucleic acid amplification and detecti...
Real-time PCR combines PCR amplification and detection into a single step. This eliminates the need to detect products using gel electrophoresis, and more importantly it enables the method to be truly quantitative. With real-time PCR, fluorescent dyes are used to label PCR products d...
ABI实时荧光定量PCR仪操作手册 Fast Real-Time PCR System Allelic Discrimination.pdf,Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Allelic Discrimination Introduction Getting Started Guide Designing an AD Experiment Setting Up the Reaction Pla
Real-Time PCR (qPCR) primer design using free online software Real-time PCR (quantitative PCR or qPCR) has become the preferred method for validating results obtained from assays which measure gene expression profiles... B Thornton,C Basu - 《Biochem Mol Biol Educ》...
Real-time PCR Support Center One resource for all your real-time PCR support needs. Navigate through the qPCR application areas to obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday qPCR problems. ...
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate ...