This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. Use the default settings to get the results in seconds. Customize any criterion to optimize the results. Orga
(qPCR) Primer Design Using Free Online Software Received for publication, May 27, 2010, and in revised form, July 30, 2010 Brenda Thornton and Chhandak Basu† School of Biological Sciences, University of Northern Colorado, Greeley, Colorado 80639 Real-time PCR (quantitative PCR or qPCR) has...
TECHNOLOGY FEATURE Hairpins and more hairpins Standards, standards Box 1: Some like it hot, some fast Box 2: Getting primed and probed Box 3: Thermocyclers go real time 308 310 306 309 310 Prime time for real-time PCR Real-time PCR is the favored method for measuring gene expression. ...
Real-Time PCR Troubleshooting Tool What is UNG/UDG? Avoiding Contamination Calculating Primer and Probe Concentrations Why TaqMan Assay Design is Important Efficiency of Real-Time PCR What is multiplexing? Taq Talk Video Series Gene Expression Learning Center miRNA Analysis Re...
Overall, the GenScript Real-time PCR Primer and Probe Design Tool is a powerful and user-friendly online tool that can help researchers design high-quality TaqMan primers and probes for real-time PCR experiments, saving time and valuable resources in the experimental process....
Real-time PCR (qPCR) primer design using free online software. Biochem. Mol. Biol. Educ. A Bimon. Publ. Int. Union Biochem. Mol. Biol. 39, 145e154.Thornton B, Basu C. Real-Time PCR (qPCR) primer design using free online software. Biochem Mol Biol Educ 2011;39:145-54....
From Ct analysis of your real-time PCR gene expression experiments, to allele cluster analysis of TaqMan® SNP Assay data, to CNV copy number determination, to HRM-curve generation, we create software that enables you to easily analyze data from
New Applied Biosystems Real-Time PCR Analysis Modules. Fast, powerful web browser-based analysis. See them now Applied Biosystems gene expression analysis DataAssist Software DataAssist™ Software is a free, easy-to-use data analysis tool that utilizes the Comparative Ct (ΔΔCt) method to rapid...
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Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of …more Experimental...