To evaluate antibody accuracy, it was tested on HEp-2 cells with permeable nucleus membranes fixed on slides. The antibody detected NuMA protein in the nuclei of HEp-2 cells, which means it can have diagnostic value in the bladder cancer patients. The truncated NuMA is...
The first method to enhance the 1270 nm phosphor signal is to use a NIR photomultiplier tube (PMT) or an InGaAs linear array in combination with scanning of the excitation laser beam, and the second one is based on the use of a NIR camera with a filter. Because the lifetime of ...
Using a recombinant mouse-human chimeric monoclonal antibody (mAb) against the main coat protein and primary immunogen of Ad5 (hexon), called 9C12 (9C12-WT), we introduced mutations L234A/L235A (LALA) to prevent Fc gamma receptor (FcγR) binding (Wines et al., 2000Wines B.D. Powell...
This autoreactive T‐cell response correlated widely with disease activity and LKM‐1 antibody status and seemed to be regulated by anticlonotypic T cells. 展开 DOI: 10.1002/HEP.510240619 年份: 1996 收藏 引用 批量引用 报错 分享 全部来源 求助全文 Semantic Scholar ...
The next day, the membrane was removed, washed well with TBS-Tween, and incubated with an HRP-labeled secondary antibody (1:5000) for 2 h at room temperature. The protein bands were visualized with a ChemiDoc Imaging System (Bio-Rad, USA). Finally, ImageJ software was used to measure ...
This process produced serum autoantibodies to a breadth of self-antigens, leading to antibody deposition in the kidneys. Our data provide insight into the maturation of the self-reactive B cell response, contextualizing the epitope spreading observed in autoimmune disease. Graphical Abstract Download: ...
A self- adjuvanting multiepitope immunogen that induces a broadly cross- reactive antibody to hepatitis C virus. Hepatology 45(4):911-920.Torresi J,Stock O M,Fischer A E,et al.A self-adjuvanting multiepitope immunogen that induces a broadly cross-reactive antibody to hepatitis C virus. ...
The main objective of the current study was to develop a universal method for a protein binding assay of complicated herbal components, and to investigate ... Y Liang,YY Zhou,YN Liu,... - 《Chinese Journal of Natural Medicines》 被引量: 9发表: 2013年 Modulation of Anti-HLA antibody produ...
The absence of the surrogate light chain, which assembles with antibody heavy chain forming a pre-BCR, leads to production of antinuclear antibodies (ANAs). Here we show that the naive follicular B-cell pool is enriched for cells expressing prototypic ANA heavy chains in these mice in a non...
Δ m/z: monoisotopic mass addition upon conjugation to a primary amine. Glc = glucose, Rha = rhamnose, GlcA = glucuronic acid, Hep = heptose, Man = mannose, Gal = galactose; # Conjugation of linker molecule and subsequent hydrolysis of ...