(F) ELISA of monoclonal antibodies against structurally distinct targets and the HEp-2 cell line lysate. ED38 was used as a positive control and the GD01 antibody as a negative control. In (A), comparison between the test antibodies and the isotype control was performed using one-way ANOVA...
Finally, the targeted effect of FA was observed by a fluorescence microscope (Leica) at the red channel. 2.1.8. Transfection efficiency and knockdown effect of the genetic nanomedicine To analyze the transfection efficiency of the siRNA in the PLGA nanomedicine, 2 × 105 of HepG2 and Huh7 ...
As a result, the C-terminal lysine in any tryptic peptide had to be unmodified in order to be cleaved. This facilitated the assignment of the actual conjugation site in particular as for most glycopeptides only one lysine residue remained as a possible site of conjugation. On the other hand...
The latter are of particular concern as they involve germinal centre (GC) formation, secondary structures that support B-cell clonal expansion, alter antibody effector function and antigen affinity, and result in immunological memory that can last for a lifetime8–12. In autoimmune diseases, and ...
Celastrol induced ROS accumulation, G2-M phase blockage, apoptosis and necrosis in H1299 and HepG2 cells in a dose-dependent manner. N-acetylcysteine (NAC), an antioxidative agent, inhibited celastrol-induced ROS accumulation and cytotoxicity. JNK phosphorylation induced by celastrol was suppressed ...
Briefly, primary mouse hepatocytes, neonatal rat cardiomyocytes, and HepG2 cells, prepared as above in 96-well plates, were treated with Na2S4 in serum-free medium and cultured at 37°C in a humidified atmosphere with 5% CO2. Untreated cells were used as control. After 24 h, cells were ...
IgGg protein levels (a) and mRNA levels (b) were detected. (c) The viability of similarly treated cells was analyzed with cell proliferation assay. Results are presented as percentage of cells proliferation in comparison to the negative control. HEp-2 and PC3 cells were also treated with the...
A, Huh7, BEL7402, and HepG2 cells were treated with the indicated concentrations of tetrandrine for 24 h. Then, the cell lysates were subjected to Western blotting using antibodies against pro-poly(ADP-ribose) polymerase, pro-CASPASE-8, pro-CASPASE-3, and LC3. A GAPDH antibody was used ...
Therefore, our findings represent a novel effective therapeutic strategy for tumour treatment. Materials and Methods Cell lines and cell culture The human hepatoma cell lines (BEL7402 and FHCC98), hepatoblastoma cell line (HepG2) and immortalised nonmalignant cell lines (L02 and HBL100) were ...
HSC were co-cultured with C34, E47, or HepG2 cells overexpressing CYP3A4 for 24 h. 10 μg of HSC extract were resolved on a 5% SDS-polyacrylamide gel followed by Western blot using rabbit monoclonal anti-collagen I antibody. The HSC/CYP3A4 co-culture had a 2-fold increase in collagen...