Hepatitis is a general term that means inflammation (irritation and swelling) of the liver. Inflammation of the liver can result from infection, exposure to alcohol, certain medications, chemicals, poisons, or from a disorder of the immune system. Hepatitis A virus (HAV) is one of several viru...
Nonreactive (negative). This means you're not currently infected with hep C. If you think you've been exposed within the last 6 months, you will need to be tested again to make sure because it can take a while for your antibody levels to rise high enough to be detected on a test....
Hepatitis C infection is diagnosed with several blood tests. The hepatitis C antibody test checks for antibodies (immune particles) that fight the virus. A "non-reactive" result means that antibodies to the virus are not detected. A "reactive" result means antibodies to the virus are present, ...
Hepatitis C infection is diagnosed with several blood tests. The hepatitis C antibody test checks for antibodies (immune particles) that fight the virus. A "non-reactive" result means that antibodies to the virus are not detected. A "reactive" result means antibodies to the virus are present, ...
Subsequently, the slides were washed with 1× PBS (3 times for 3 min, at static condition and at room temperature), and incubated with secondary biotinylated goat anti-rabbit antibody for 1 h at static condition at 37 °C. The slides were then processed according to the manufacturer's ...
The kit also includes buffers for Compound Dilution, Fixation, Cell Permeabilization, Antibody Dilution and Washing, as well as disposable syringes and needles for HepG2 cell handling and plate sealers. All materials and protocols provided have been validated for multiplexed analysis on both the GE ...
To evaluate antibody accuracy, it was tested on HEp-2 cells with permeable nucleus membranes fixed on slides. The antibody detected NuMA protein in the nuclei of HEp-2 cells, which means it can have diagnostic value in the bladder cancer patients. The truncated NuMA is ...
After that cells were again washed and incubated with an appropriate fluorescence-conjugated secondary antibody at room temperature. Finally, the cells were mounted with fluorescent medium, covered with glass cover slip and observed under a fluorescent microscope [22]. 2.10. Immunoblotting HepG2 cells...
HepG2 cell lysate was prepared as described in the RNA pull-down assay section. Cell lysates from 1 × 106cells were immunoprecipitated using 2 μg eIF4A antibody or IgG at 4 °C overnight with rotation. The immunoprecipitates were digested with proteinase K (Thermo Scientific), an...
L-Arginine (Arg) is a versatile amino acid that plays crucial roles in a wide range of physiological and pathological processes. In this study, to investigate the alteration induced by Arg supplementation in proteome scale, isobaric tags for relative and absolute quantification (iTRAQ) based proteom...