基于mDRIP-seq,研究团队对多个物种样本R-loop水平进行了定量评估和比较,并发现不同物种R-loop水平的差异一定程度上可以由基因密度的差异解释(图2b和2c)。进一步,研究团队利用该技术首次对近100种解旋酶活性和组蛋白修饰活性相关基因缺失的...
Abstract R-loops are functional structures in chromatin comprising one single-stranded DNA and a DNA:RNA hybrid. Here, we report ssDRIP-seq, a single-strand DNA ligation-based library preparation technique for genome-wide identification of R-loops. When applied inArabidopsis, ssDRIP-seq exhibits ...
12. Sun, Q., Csorba, T., Skourti- Stathaki, K., Proudfoot, N. J. & Dean, C. R- loop stabilization represses antisense transcription at the Arabidopsis FLC locus. Science 340, 619–621 (2013). 13. Powella etal., R-loop formation at Snord116 mediates topotecan inhibition of Ube3a...
2023年8月11日,清华大学生命学院孙前文实验室在《科学·进展》(Science Advances)杂志上发表了题为“DDM1介导的R-loop解除和H2A.Z排斥促进拟南芥异染色质的形成”(DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in Arabidopsis)的研究论文4,揭示了拟南芥中染色质重塑蛋白DD...
2020年1月7日,清华-北大生命科学联合中心、清华大学生命学院孙前文实验室在Cell Reports杂志在线发表题为“RHON1共转录移除R-loop以维持拟南芥叶绿体基因组稳定性 (RHON1 co-transcriptionally resolves R-loops for Arabidopsis chloroplast genom...
来自清华大学生科院,清华-北大生命科学联合中心的研究人员发表了题为“The R-loop is a common chromatin feature of the Arabidopsis genome”的文章,首次报道了精准、高效、高通量检测R-loop的方法,并利用此方法首次揭示模式植物拟南芥基因组中R-loop的分布特征。
清华大学生科院,清华-北大生命科学联合中心的研究人员发表了题为“The R-loop is a common chromatin feature of the Arabidopsis genome”的文章,首次报道了精准、高效、高通量检测R-loop的方法,并利用此方法首次揭示模式植物拟南芥基因组中R-loop的分布特征。
2020年1月7日,清华-北大生命科学联合中心、清华大学生命学院孙前文实验室在Cell Reports杂志在线发表题为“RHON1共转录移除R-loop以维持拟南芥叶绿体基因组稳定性 (RHON1 co-tranionally resolves R-loops for Arabidopsis chloroplast genome maintenance)”的研究论文。
5. Q. Sun, T. Csorba, K. Skourti-Stathaki, N. J. Proudfoot, C. J. S. Dean, R-loop stabilization represses antisense transcription at the Arabidopsis FLC locus. 340, 619-621 (2013). 6. T. Yasuhara et al., Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcrip...
Another non-canonical nucleic acid structure called an R-loop or RNA:DNA structure is preferentially formed within G-rich sequences and possesses greater thermodynamic stability than the original DNA:DNA duplex6. R-loops have been experimentally observed in a wide range of organisms, from bacteria ...