pulse-chase assay 青云英语翻译 请在下面的文本框内输入文字,然后点击开始翻译按钮进行翻译,如果您看不到结果,请重新翻译! 翻译结果1翻译结果2翻译结果3翻译结果4翻译结果5 翻译结果1复制译文编辑译文朗读译文返回顶部 脉冲追逐检测的 翻译结果2复制译文编辑译文朗读译文返回顶部...
因此,有硫醇反应性修饰的生物素,你可以将其与含有4-硫基的RNA结合。所以这里的基本思想是,你可以在4-硫基中培养细胞数代,使RNA完全结合4-硫基,而不是尿嘧啶。细胞在这种情况下是正常的,它们并不太在乎。 然后,当时间等于零时,你会加入大量过量的尿嘧啶。所以通常,我相信在这篇论文的实验中,他们使用了一微...
求翻译:pulse-chase assay是什么意思?待解决 悬赏分:1 - 离问题结束还有 pulse-chase assay问题补充:匿名 2013-05-23 12:21:38 脉冲追逐检测的 匿名 2013-05-23 12:23:18 脉冲的大通银行成分檢定 匿名 2013-05-23 12:24:58 脉冲追逐分析用试样 匿名 2013-05-23 12:26:38 脉冲大通测定 匿...
In this review article, we focus on a long-established technique to investigate MHC class I cell surface transport: the radioactive pulse-chase assay. We describe the design of experiments and the evaluation of the data, and we point out its potential for future exploration of class I, as ...
The assay consists of two parts: a pulse of DNA analog [bromodeoxyuridine (BrdU) or tritiated nucleotide, for exam- ple] followed by a chase period. During the pulse, all prolif- erating cells are labeled. During the chase, the analog is absent, so fast cycling cells will dilute the ...
A pulse‐chase strategy for EdU labelling assay is able to rapidly quantify cell division orientation Summary Measurement of the direction of cell division is an important, yet difficult, task to analyse how a plant organ acquires its final shape from an in... X Yin,H Tsukaya - 《New Phyto...
Thus, to minimize variance, all samples should be measured in triplicate and, whenever possible, all samples for a given experiment should be measured within a single assay. Perform the ‘click’ reaction on the sample homogenates Timing: 75–90 min The ‘click’ reaction will covalently bind ...
In this review article, we focus on a long-established technique to investigate \\{MHC\\} class I cell surface transport: the radioactive pulse-chase assay. We describe the design of experiments and the evaluation of the data, and we point out its potential for future exploration of class ...
These time-slicing methods provide powerful tools to assay and quantify the fate and turnover rate of proteins of different ages. We cover advantages and pitfalls of SNAP-tagging in fixed- and live-cell studies and evaluate the recently developed fast-acting SNAPf variant. In addition, to ...
所以基本上,脉冲追踪分析背后的想法是标记分子。通常,当你这样做时,你会想用一些东西来完全标记它们,然后停止快速标记它们。你也可以用另一种方式来做,但那样更难。只需给它们贴上完整的标签,然后添加一些东西,这样它们就不会再被贴上标签了,这就是这样做的。