Firstly,we adapted EDU(5-ethynyl-2'- deoxyuridine)pulse-chase assay with dose(10 mg/kg)reported in the literature,and the most embryo were not labeled by EDU. Following, we optimized the dosage and pulse time by analyzing the labeling effects of EDU in SHF cells and the migration of EDU...
今年8月份发表在《PNAS》期刊上的文章中,通过每天腹腔注射5 mg/kg EdU(RiboBio)到携带肿瘤的裸鼠体内,连续注射2周后,分离LSC1和LAC1细胞,检测裸鼠体内肿瘤干细胞的不对称和对称分裂情况。 (A) EdU pulse–chase experiment in vivo. (B) Representative IF images of asymmetric and symmetric cell division in ...
摘要: Imaging or quantifying protein synthesis in cellul 关键词: cell cycle click chemistry 5-ethynyl-2-deoxyuridine (EdU) fluorescent proteins G1 subcompartments Golgi synthesis Kaede pulse-chase tobacco (Nicotiana tabacum) BY2 cells 年份: 2015 ...
Pulse-chase experiments (脉冲追踪试验):将细胞与放射性标记的合成底物(属于某些途径或大分子)一起培养,则标记结果将 在下一步与非标记底物共培养中延续。 Q Quaternary structure of protein (蛋白质四级结构):指蛋白质的多亚基组成。 Quick-stop dna mutant (快停突变体):当温度升高到 42℃时大肠杆菌 DNA ...
【题目】1.Pathway of Atoms in FermentationA "pulse-chase" experiment using 14C-labeledcarbon sources is carried out on a yeast extract maintained under strictly anaerobic conditions to produce ethanol.T he experiment consistsof incubating a small amount of 14C -labeledsubstrate (the pulse)with the...
(A) EdU pulse–chase experiment in vivo. (B) Representative IF images of asymmetric and symmetric cell division in vivo. The percentages of EdU asymmetry (Asy) or symmetry (Sym) in LSC1 and LAC1 cells after IGF1 (500 ng/mL) or PBS treatment were summarized in Right. ...
Pulse-chase experiments (脉冲追踪试验):将细胞与放射性标记的合成底物(属于某些途径或大分子)一起培养,则标记结果将 在下一步与非标记底物共培养中延续。 Q Quaternary structure of protein (蛋白质四级结构):指蛋白质的多亚基组成。 Quick-stop dna mutant (快停突变体):当温度升高到 42℃时大肠杆菌 DNA ...