medium is highly complex and requires the addition of fetal bovine serum (FBS). FBS is a natural product of undefined composition and variable concentration of metabolites. Thus, a complete and monoisotopic labeling of nucleosides and even nucleobases for a pulse chase NAIL-MS assay is challenging...
Pulse-chase experiments. MG1655/pKW332549c was grown exponentially in synthetic M9 medium containing all amino acids, except methionine and 0.5% glycerol as the carbon source. At OD450 ¼ 0.3–0.4, the cultures were induced with 0.2% arabinose. Samples (0.5 ml) were pulsed for 1 min with...
D, graph representing the band intensity ratios between 28 S rRNA and 32 S pre-rRNA observed during the pulse-chase shown in C. The values are the means ± S.D. obtained from three independent experiments. The difference of the mean values at 6 h is statistically significant (p < 0.05,...
of isolated nucleoli in the nucleolar-enriched samples (Supplementary Fig.1a). In addition, chromatin immunoprecipitation coupled to qPCR (ChIP) revealed the localization of TBPL1 and PAF1 across the IGS (Fig.1i, j), and this was comparable to other genetic loci known to be occupied by these...
SLFN13-N cleaves native tRNA and rRNA.a,bCleavage assay of hSLFN13-N (a) and rSLFN13-N (b) on native tRNAs in the presence of Mg2+. Small RNA-293T denotes total small RNA extracted from 293T cells that is mainly constituted of tRNAs. tRNASerand tRNAGlywere used as controls to ind...