pulse-chase assay选择语言:从 到 翻译结果1翻译结果2 翻译结果3翻译结果4翻译结果5 翻译结果1复制译文编辑译文朗读译文返回顶部 脉冲追逐检测的 翻译结果2复制译文编辑译文朗读译文返回顶部 脉搏追赶分析 翻译结果3复制译文编辑译文朗读译文返回顶部 脉冲大通测定 翻译结果4复制译文编辑译文朗读译文返回顶部 脉冲的大通...
因此,有硫醇反应性修饰的生物素,你可以将其与含有4-硫基的RNA结合。所以这里的基本思想是,你可以在4-硫基中培养细胞数代,使RNA完全结合4-硫基,而不是尿嘧啶。细胞在这种情况下是正常的,它们并不太在乎。 然后,当时间等于零时,你会加入大量过量的尿嘧啶。所以通常,我相信在这篇论文的实验中,他们使用了一微...
待解决 悬赏分:1 - 离问题结束还有 pulse-chase assay问题补充:匿名 2013-05-23 12:21:38 脉冲追逐检测的 匿名 2013-05-23 12:23:18 脉冲的大通银行成分檢定 匿名 2013-05-23 12:24:58 脉冲追逐分析用试样 匿名 2013-05-23 12:26:38 脉冲大通测定 匿名 2013-05-23 12:28:18 脉搏...
Most commonly, autophagic flux is measured using a metabolic pulse-chase (MPC) assay, which labels a subset of proteins ("pulse") and then measures levels of these proteins at regular intervals ("chase") to estimate the mean half-life of proteins cleared by autophagy. In this chapter, we ...
The assay consists of two parts: a pulse of DNA analog [bromodeoxyuridine (BrdU) or tritiated nucleotide, for exam- ple] followed by a chase period. During the pulse, all prolif- erating cells are labeled. During the chase, the analog is absent, so fast cycling cells will dilute the ...
A pulse‐chase strategy for EdU labelling assay is able to rapidly quantify cell division orientation Summary Measurement of the direction of cell division is an important, yet difficult, task to analyse how a plant organ acquires its final shape from an in... X Yin,H Tsukaya - 《New Phyto...
Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (‘pulse’) of a kinase of interest, as well as global and in-depth characterization (‘chase’) of the perturbed proteome under living conditions. Here we developed an optogenetic ‘pulse-chase’ ...
A pulse-chase strategy for EdU labelling assay is able to rapidly quantify cell division orientation.EdU标签检测脉冲追踪策略能够迅速量化细胞分裂方向 Is capsulectomy a feasible and useful measure in internal pulse generator replacement procedures A technical note on the.capsulectomy是可行的和有用的测量...
Using optical pulse-chase labeling (OPL) methodology we identified that both soluble and insoluble, phosphorylated WT and mutant A53T human α-syn, show similar rates of production and turnover to Dendra2 alone. However, upon addition of α-syn PFFs some neurons show a slowing of α-syn ...
More importantly, pulse-chase analysis of these proteins was successfully carried out by appropriate protein modification using LDAI chemistry. The labeling-based biochemical assay allowed us to determine the protein half-life under almost natural cellular context. Furthermore, their degradation pathways ...