1992. Comparative studies of protein biosynthesis: the main experimental parameters in pulse and pulse- chase experiments must be standardized. Biol Cell 74:235 - 238.Appel F., Couderc M. and Feger E. 1992. Compara tive studies of protein biosynthesis: the main experimental parameters in pulse...
网络脉冲追踪试验;脉冲追踪实验 网络释义
pulse-chase labeling实验原理Pulse-chase labeling实验原理是通过给予细胞标记性核苷酸脉冲,然后在 chase 阶段监测和分析细胞内标记的分布和保留,以研究细胞周期、蛋白质合成和半衰期等生物学过程。©2022 Baidu |由 百度智能云 提供计算服务 | 使用百度前必读 | 文库协议 | 网站地图 | 百度营销 ...
The meaning of PULSE-CHASE is involving the exposure of cells to a substrate bearing a radioactive label for a predetermined time followed by exposure to a high concentration of unlabeled substrate in order to stop uptake of the labeled substrate and fol
We show in pulse-chase experiments that the C-terminal fragments, CT, generated by alpha-secretase and A4CT, generated by beta-secretase, could be generated from immature full-length APP before O-glycosylation is completed. Thus beta A4 may be generated from immature APP that has not passed ...
Therefore, we studied the biosynthesis and maturation of the interleukin-6 receptor and its signaling subunit gp130 by pulse chase experiments in stably ... C Gerhartz,E Dittrich,T Stoyan,... - 《European Journal of Biochemistry》 被引量: 111发表: 1994年 Biogenesis of membrane-bound and secre...
将细胞与放射性标记的合成底物(属于某些途径或大分子)一起培养，则标记结果将在下一步与非标记底物共培养中延续。通过对放射性物质的检测就可以追踪你的实验结果。
Pulse-chase experiments have proved to be a powerful tool to study protein folding, maturation, and degradation in mammalian cells. When short pulses are applied, a fraction of the total protein pool can be followed from synthesis to degradation in its natural environment.DOI...
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