We show that such limited pulses of receptor expression lead to signaling phenotypes that more closely reflect endogenous signaling events. Copyright 2016 Elsevier Inc. All rights reserved.doi:10.1016/bs.mcb.2015.11.011Wargachuk RDevost DZhou CHébert TEMethods Cell Biol...
- 《Methods in Molecular Biology》 被引量: 44发表: 2009年 Smurf1 directly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathway. Pulse-chase labeling and a ubiquitination experiment using MG132, a proteasomal inhibitor, ...
Detailed cell progression could be identified with the present method more dynamically than with DNA histograms or conventional BrdU-pulse-chase methods, and two different growth statuses could also be distinguished. Therefore, this two-cell-divisions-BrdU-pulse-chase method may facilitate the analysis ...
wt. were detected in cells infected with Semliki Forest virus by use of pulse-chase methods in... MJ Morser,DC Burke - 《Journal of General Virology》 被引量: 141发表: 1974年 Mechanism for Control of Synthesis of Semliki Forest Virus 26S and 42S RNA When cells infected with the Semliki...
depend on the type of proteins and the existing conditions (Ciechanover, 2005). Therefore, valuable methods such as pulse-chase analysis or imaging are greatly desirable for investigating membrane-bound proteins in intact cellular contexts. Furthermore, such methods require the general applicability ...
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Methods Mol BiolTakahashi, M., and Ono, Y. (2003) Pulse-chase analysis of protein kinase C. Methods Mol Biol 233: 163-170.Takahashi, M. , and Ono, Y. ( 2003 ) Pulse-chase analysis of protein kinase C . Methods Mol Biol 233 : 163 – 170 ....
Hou T, Rinderknecht CH, Hadjinicolaou AV, Busch R, Mellins E. Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading. Methods Mol Biol. 2012; 960:411-32. PMID: 23329504.Hou T, Rinderknecht CH, Hadjinicolaou AV, Busch R, Mellins E. Pulse-chase...
Methods Mol Biol 233 : 163 – 170 .Takahashi, M., and Ono, Y. (2003) Pulse-chase analysis of protein kinase C. Methods Mol Biol 233: 163-170.Takahashi, M. and Y. Ono (2003). "Pulse-chase analysis of protein kinase C." Methods in Molecular Biology 233: 7....
While such methods are invaluable for defining the variations in protein concentrations which follow the perturbation of a biological system, they do not offer information on the mechanisms underlying such changes. Expanding on previous work [1], we developed a pulse-chase (pc) variant of SILAC (...