Pulse and Pulse Chase Analysis of Viral Replication Forks Reveals Interaction Dynamics Between Nascent Viral DNA and Associated Proteins.Jill, A. DembowskiSarah E., DremelNeal, A. DeLuca
Chase incubation with normal supplemented DMEM was similarly preceded with wash steps. Medium changes for SILAC pulse and chase incubations began at a time judged to permit the conclusion of incubations before cells reached 90% confluence. Every experiment was repeated 2–4 times with technical ...