Pulse-chase experiment for nuclear incorporation of BrdU.Sushmita, RoySaleem, JavedSwatantra, K. JainSubeer, S. MajumdarAsok, Mukhopadhyay
The meaning of PULSE-CHASE is involving the exposure of cells to a substrate bearing a radioactive label for a predetermined time followed by exposure to a high concentration of unlabeled substrate in order to stop uptake of the labeled substrate and fol
网络脉冲追踪实验;冈崎的实验-脉冲追踪实验 网络释义
Labeling with stable isotopes enables mass spectrometric detection of the heavy:light or H:L ratio of a large fraction of the mitochondrial proteome as an index of the synthesis rate for a far greater number of proteins than in a radioactive labeling experiment. When labeling is done on non...
The labeling experiment was conducted almost in the same manner as in the case of CA12; that is, the labeling reagent was mixed with HEK293 cells expressing B2R, followed by incubation for 3 hr. The B2R labeling was analyzed by WB analysis using SAv-HRP and anti-B2R after the cell ...
Carbon sources of Antarctic nematodes as revealed by natural carbon isotope ratios and a pulse-chase experiment. Polar Biology 31 (1), 1-13.Moens, T., Vanhove, S., De Mesel, I., Kelemen, B., Janssens, T., Dewicke, A., Vanreusel, A., 2007. Carbon sources of Antarctic nematodes ...
a PPM-based communication protocol was integrated into the software system. A verification experiment demonstrates the functionality of the developed system for stress wave communication using piezoceramic transducers and the result shows that the data transmission speed of this new communication system can...
During the experiment, each participant fully understood the research content of the project and volunteered to participate in the data collection. The specific inclusion criteria for the subjects are shown below: 1. In good physical condition and no cold symptoms; 2. Good heart condition without...
experienced a choking sensation during these moments. At first, Mrs. Thomas interpreted the memories as indication that she was being choked by hands or that she was being prevented from calling out to her friends; later, however, she came to the tentative conclusion that an experiment was ...
Pulse-chase experiment using the doxycycline-inducible H2B-GFP system.doi:10.1371/journal.pone.0040691.g001Wang YongyiSacchetti AndreaR. Matthijsvan der Zee MartenH. PaulJoosten RosalieW. B. CurtJ. AntonGrootegoedJ. LeenFodde Riccardo