Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.Highlights
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleoti...
0 文档热度: 文档分类: 待分类 系统标签: 序列载体laczpucampicillincloning pUC19DNAPositionsofvariouselementsVectorsize(bp)2686Multiplecloningsite396-452LacZα-peptide146-469Ampicillinresistancegene1626~2486pUCorigin867-1455primerbindingsites:BcaBESTSequencingPrimerM13-47bindingsite352-375BcaBESTSequencingPrimerRV-...
产品类型:质粒 品牌:Thermo Fisher 货号:SD0061 规格:50μg 价格:609 产品介绍 技术参数 相关产品 产品介绍 描述 Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is...
multiple cloning site" primer_bind complement(291..308) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(291..307) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(300..322)...
The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain a multiple cloning site at thelacZ'region that enables recombinant plamids to be verified via blue/white colony screening using ...
This plasmid vector also contains important features. It has a N terminal fragment ofβ glactosidase (lacz) gene. It also has a multiple cloning site (MCS) region. This region is split into codons 6 and 7 of lacz gene. MCS region provides many restriction endonucleases restriction sites. ...
One pair of expression vectors, pARO180 and pARO190, retains the β-lactamase ( bla) gene and twelve of the 13 restriction enzyme multiple cloning sites (MCS) of pUC18/19. Another pair was created by replacing the bla gene with the gene encoding kanamycin resistance ( kan) from Tn5. The...
and the vector also contains more than 10 multiple cloning sites, so a user can conveniently select suitable restrictive endonuclease to research the action site of intergrase; and the vector has aminobenzyl resistance, is convenient to screen, can be used to carry out in vitro detection of the...
one of multiple similar variants" misc_feature 396..452 /note="MCS" /note="pUC18/19 multiple cloning site" primer_bind complement(465..481) /note="M13 rev" /note="common sequencing primer, one of multiple similar 2 variants" protein_bind 489..505 /bound_moiety="lac repressor encoded by...