pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleoti...
Thermo Scientific pUC19 vector is a small, high copy number,E. coliplasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation. Highlights • Purified by chromatography using proprietary patented technology ...
The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain a multiple cloning site at the lacZ' region that enables recombinant plamids to be verified via blue/white colony screening ...
multiple cloning site" primer_bind complement(291..308) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(291..307) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(300..322)...
pUC19DNA Positionsofvariouselements Vectorsize(bp)2686 Multiplecloningsite396-452 LacZα-peptide146-469 Ampicillinresistancegene1626~2486 pUCorigin867-1455 primerbindingsites: BcaBESTSequencingPrimerM13-47bindingsite352-375 BcaBESTSequencingPrimerRV-Mbindingsite478-501 ...
Pfeiffer: 1062/3 'AT' to 'TA' to match revised sequence of PBR322 The beta-galactosidase mRNA sequence including the multiple cloning site of M13mp19 is on the strand complementary to that shown. KEYWORDS CROSSREFERENCE #complement VecBase(3):pUC19 #prerevised GenBank(50):M11662, EMBL(11...
and the vector also contains more than 10 multiple cloning sites, so a user can conveniently select suitable restrictive endonuclease to research the action site of intergrase; and the vector has aminobenzyl resistance, is convenient to screen, can be used to carry out in vitro detection of the...
It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.Highlights• Purified by chromatography using proprietary patented technology• More than 90% in the supercoiled form• Isolated from E.coli (dam+, dcm+)• For pUC18 DNA ...
It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.Highlights• Purified by chromatography using proprietary patented technology• More than 90% in the supercoiled form• Isolated from E.coli (dam+, dcm+)• For pUC18 DNA ...
It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.Highlights• Purified by chromatography using proprietary patented technology• More than 90% in the supercoiled form• Isolated from E.coli (dam+, dcm+)• For pUC18 DNA ...